ArticleYeast (Chichester, England)2017
Saccharomyces cerevisiae Atf1p is an alcohol acetyltransferase and a thioesterase in vitro.
Article in Yeast (Chichester, England), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Multi-layered metabolic remodeling ofMetabolic engineering communications · 2026Article
- An acetyltransferase family required for biosynthesis of diverse marine carotenoids.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Identification and Sensory Evaluation of 3-Sulfanylhexyl Propionate and 3-Sulfanylhexyl Butyrate in Wine.Journal of agricultural and food chemistry · 2026Article
- Screening of flavor-enhancing yeast and its role in fig fermentation.Frontiers in bioengineering and biotechnology · 2026Article
- Endogenous pathway dysregulation in Kluyveromyces marxianus under nitrogen limitation drives 2-phenylethyl acetate and 2-phenylethanol overproduction.Scientific reports · 2025Article
- Towards biotechnological production of bio-based low molecular weight esters: a patent review.RSC advances · 2024Review
- Metabolic engineering of Escherichia coli for high-level production of benzyl acetate from glucose.Microbial cell factories · 2024Article
- Production of Esters inMicroorganisms · 2024Article
- The ester production capacity of Pichia kudriavzevii based on functional annotation of genes.World journal of microbiology & biotechnology · 2023Article
- Microbial production of high octane and high sensitivity olefinic ester biofuels.Biotechnology for biofuels and bioproducts · 2023Article
- Key enzymes involved in the utilization of fatty acids byFrontiers in microbiology · 2023Review
- Metabolic engineering of Clostridium autoethanogenum for ethyl acetate production from CO.Microbial cell factories · 2022Article
- Engineering Saccharomyces cerevisiae for production of the capsaicinoid nonivamide.Microbial cell factories · 2022Article
- Thioesterase enzyme families: Functions, structures, and mechanisms.Protein science : a publication of the Protein Society · 2022Article
- Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate.Microbial cell factories · 2022Article
- Microbial production of butyl butyrate: from single strain to cognate consortium.Bioresources and bioprocessing · 2021Review
- Molecular characterization of the Saccharomycopsis fibuligera ATF genes, encoding alcohol acetyltransferase for volatile acetate ester formation.Journal of microbiology (Seoul, Korea) · 2021Article
- Development of Genetic Modification Tools forInternational journal of molecular sciences · 2021Article
- From Eat to trEat: engineering the mitochondrial Eat1 enzyme for enhanced ethyl acetate production inBiotechnology for biofuels · 2020Article
- Multilevel optimisation of anaerobic ethyl acetate production in engineeredBiotechnology for biofuels · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The alcohol-O-acyltransferases are bisubstrate enzymes that catalyse the transfer of acyl chains from an acyl-coenzyme A (CoA) donor to an acceptor alcohol. In the industrial yeast Saccharomyces cerevisiae this reaction produces acyl esters that are an important influence on the flavour of fermented beverages and foods. There is also a growing interest in using acyltransferases to produce bulk quantities of acyl esters in engineered microbial cell factories. However, the structure and function of the alcohol-O-acyltransferases remain only partly understood. Here, we recombinantly express, purify and characterize Atf1p, the major alcohol acetyltransferase from S. cerevisiae. We find that Atf1p is promiscuous with regard to the alcohol cosubstrate but that the acyltransfer activity is specific for acetyl-CoA. Additionally, we find that Atf1p is an efficient thioesterase in vitro with specificity towards medium-chain-length acyl-CoAs. Unexpectedly, we also find that mutating the supposed catalytic histidine (H191) within the conserved HXXXDG active site motif only moderately reduces the thioesterase activity of Atf1p. Our results imply a role for Atf1p in CoA homeostasis and suggest that engineering Atf1p to reduce the thioesterase activity could improve product yields of acetate esters from cellular factories. © 2017 The Authors. Yeast published by John Wiley & Sons, Ltd.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.