Evidence map›Paper›PMID 28159817›Full record

ArticleJournal of the American Heart Association2017

Tissue Transglutaminase Modulates Vascular Stiffness and Function Through Crosslinking-Dependent and Crosslinking-Independent Functions.

Jochen Steppan, Yehudit Bergman, Kayla Viegas, Dinani Armstrong, Siqi Tan, Huilei Wang, Sean Melucci, Daijiro Hori, Sung Yong Park, Sebastian F Barreto and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Heart Association, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
7.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 81 citations in OpenAlex.

  1. Beneficial vascular effects of oral phosphatidylserine supplementation in type 2 diabetes.Journal of applied physiology (Bethesda, Md. : 1985) · 2026
    Trial
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. LOXL2 inhibition ameliorates pulmonary artery remodeling in pulmonary hypertension.American journal of physiology. Lung cellular and molecular physiology · 2024
    Article
  8. Sex differences and role of lysyl oxidase-like 2 in angiotensin II-induced hypertension in mice.American journal of physiology. Heart and circulatory physiology · 2024
    Article
  9. Article
  10. Article
  11. Review
  12. The extracellular matrix mechanics in the vasculature.Nature cardiovascular research · 2023
    Review
  13. Review
  14. Review
  15. Role of adropin in arterial stiffening associated with obesity and type 2 diabetes.American journal of physiology. Heart and circulatory physiology · 2022
    Article
  16. Cystamine reduces vascular stiffness in Western diet-fed female mice.American journal of physiology. Heart and circulatory physiology · 2022
    Article
  17. Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 3 institutions in 3 countries.

Jochen SteppanDepartment of Anesthesiology & Critical Care Medicine, Johns Hopkins University, Baltimore, MD.
Yehudit BergmanDepartment of Anesthesiology & Critical Care Medicine, Johns Hopkins University, Baltimore, MD.
Kayla ViegasDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia.
Dinani ArmstrongDepartment of Anesthesiology & Critical Care Medicine, Johns Hopkins University, Baltimore, MD.
Siqi TanDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Huilei WangDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD.
Sean MelucciDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Daijiro HoriDepartment of Surgery, Johns Hopkins University, Baltimore, MD.
Sung Yong ParkDepartment of Anesthesiology & Critical Care Medicine, Johns Hopkins University, Baltimore, MD.
Sebastian F BarretoDepartment of Anesthesiology & Critical Care Medicine, Johns Hopkins University, Baltimore, MD.
Abraham IsakDepartment of Environmental Health Sciences, Johns Hopkins University, Baltimore, MD.
Sandeep JanduDepartment of Anesthesiology & Critical Care Medicine, Johns Hopkins University, Baltimore, MD.
Nicholas FlavahanDepartment of Anesthesiology & Critical Care Medicine, Johns Hopkins University, Baltimore, MD.
Mark ButlinDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia.
Steven S AnDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Alberto AvolioDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia.
Dan E BerkowitzDepartment of Anesthesiology & Critical Care Medicine, Johns Hopkins University, Baltimore, MD.
Marc K HalushkaDepartment of Pathology, Johns Hopkins University, Baltimore, MD.
Lakshmi SanthanamDepartment of Anesthesiology & Critical Care Medicine, Johns Hopkins University, Baltimore, MD lsantha1@jhmi.edu.
Johns Hopkins University · USMacquarie University · AUYonsei University · KR

Funding

Nrf2 as a critical determinant of smooth muscle functionR01HL107361 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI AN, STEVEN S · 2011 to 2015
$2.0M
Transglutaminase 2 S-Nitrosylation: Role in Age-Related Vascular StiffnessR01HL105296 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI BERKOWITZ, DAN E · 2010 to 2013
$1.6M
NHLBI NIH HHS R01 HL105296NHLBI NIH HHS R01 HL107361
6 · The paper itself

Abstract

backgroundThe structural elements of the vascular wall, namely, extracellular matrix and smooth muscle cells (SMCs), contribute to the overall stiffness of the vessel. In this study, we examined the crosslinking-dependent and crosslinking-independent roles of tissue transglutaminase (TG2) in vascular function and stiffness. METHODS AND

resultsSMCs were isolated from the aortae of TG2-/- and wild-type (WT) mice. Cell adhesion was examined by using electrical cell-substrate impedance sensing and PicoGreen assay. Cell motility was examined using a Boyden chamber assay. Cell proliferation was examined by electrical cell-substrate impedance sensing and EdU incorporation assays. Cell micromechanics were studied using magnetic torsion cytometry and spontaneous nanobead tracer motions. Aortic mechanics were examined by tensile testing. Vasoreactivity was studied by wire myography. SMCs from TG2-/- mice had delayed adhesion, reduced motility, and accelerated de-adhesion and proliferation rates compared with those from WT. TG2-/- SMCs were stiffer and displayed fewer cytoskeletal remodeling events than WT. Collagen assembly was delayed in TG2-/- SMCs and recovered with adenoviral transduction of TG2. Aortic rings from TG2-/- mice were less stiff than those from WT; stiffness was partly recovered by incubation with guinea pig liver TG2 independent of crosslinking function. TG2-/- rings showed augmented response to phenylephrine-mediated vasoconstriction when compared with WT. In human coronary arteries, vascular media and plaque, high abundance of fibronectin expression, and colocalization with TG2 were observed.

conclusionsTG2 modulates vascular function/tone by altering SMC contractility independent of its crosslinking function and contributes to vascular stiffness by regulating SMC proliferation and matrix remodeling.

Indexed as

AnimalsAorta, ThoracicApoptosisBlotting, WesternCell DifferentiationCell MovementCell ProliferationCells, CulturedCollagenCoronary VesselsGTP-Binding ProteinsHumansImmunohistochemistryMaleMiceModels, AnimalCollagenGTP-Binding ProteinsProtein Glutamine gamma Glutamyltransferase 2Transglutaminasescrosslinkingmatrix remodelingtissue transglutaminasevascular biologyvascular diseasevascular smooth musclevascular stiffnessvessel

Identifiers

PMID28159817
PMCPMC5523743
OpenAlexW2584373701

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.