ArticleParasites & vectors2017
Identification of microRNAs expressed in the midgut of Aedes albopictus during dengue infection.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Limited impact of the siRNA pathway on transposable element expression in Aedes aegypti.BMC biology · 2025Article
- Acute bee paralysis virus regulation of microRNA expression dynamics in the insect host (Frontiers in cellular and infection microbiology · 2025Article
- Response of the mosquito immune system and symbiotic bacteria to pathogen infection.Parasites & vectors · 2024Review
- microRNA Expression Dynamics inInsects · 2023Article
- Intrinsic factors driving mosquito vector competence and viral evolution: a review.Frontiers in cellular and infection microbiology · 2023Review
- Revised Annotation and Characterization of NovelBiology · 2022Article
- MicroRNAs and other small RNAs in Aedes aegypti saliva and salivary glands following chikungunya virus infection.Scientific reports · 2022Article
- Regulatory Role of Host MicroRNAs in Flaviviruses Infection.Frontiers in microbiology · 2022Review
- Chandipura virus dysregulates the expression of hsa-miR-21-5p to activate NF-κB in human microglial cells.Journal of biomedical science · 2021Article
- Development of miRNA-Based Approaches to Explore the Interruption of Mosquito-Borne Disease Transmission.Frontiers in cellular and infection microbiology · 2021Review
- Tropical Medicine in China: Bibliometric Analysis Based on Web of Science (2010-2019).Journal of tropical medicine · 2021Article
- Global Analysis of Small Non-Coding RNA Populations across Tissues in the Malaria Vector,Insects · 2020Article
- Evaluation of Exosomal miRNA in Blood as a Potential Diagnostic Biomarker for Human Non-Small Cell Lung Cancer.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito,Viruses · 2019Article
- Screening for differentially expressed miRNAs in Aedes albopictus (Diptera: Culicidae) exposed to DENV-2 and their effect on replication of DENV-2 in C6/36 cells.Parasites & vectors · 2019Article
- miRNAs in Insects Infected by Animal and Plant Viruses.Viruses · 2018Review
- Multifaceted functional implications of an endogenously expressed tRNA fragment in the vector mosquito Aedes aegypti.PLoS neglected tropical diseases · 2018Article
- Aedes aegypti microRNA miR-2b regulates ubiquitin-related modifier to control chikungunya virus replication.Scientific reports · 2017Article
- Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon.BMC genomics · 2017Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe midgut is the first barrier to dengue virus (DENV) infections of mosquitoes and therefore is a major bottleneck for the subsequent development of vector competence. However, the molecular mechanisms responsible for this barrier are unknown.
resultsWe constructed three small RNA libraries from the midguts of adult Aedes albopictus females that had been fed on either sugar solution, an uninfected blood meal, or a blood meal infected with DENV-2, and112 conserved microRNAs represented by 173 miRNA sequences were identified, with 34 novel microRNAs predicted by Mireap, RNAfold and Sfold software. In addition, the expression of aal-miR-1174, aal-miR-2951 and aal-miR-956 was confirmed via stem-loop quantitative real-time PCR (qRT-PCR). Compared with microRNA expression profiles of mosquitoes that had ingested a regular blood meal, 43 microRNAs were upregulated and 4were downregulated in mosquitoes that had ingested a DENV-2-infected blood meal. Among the differentially expressed microRNAs, miR-1767, miR-276-3p, miR-4448 and miR-4728-5p were verified via stem-loop qRT-PCR.
conclusionsAnalyses indicated that the changing patterns in miRNA expression during DENV-2 infection were significant and varied at different time points post infection. Most miRNA were upregulated at 24 h but were downregulated at 48 h post DENV-2 intake. The aal-miR-4728-5p was chosen for an in vitro transient transfection assay, and the results show that this miRNA enhances DENV replication in C6/36 cells. This study provides the first information on microRNAs expressed in the midgut of Ae. albopictus and describes species-specific changes in their expression levels following infection by DENV-2.
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