ArticleJournal of experimental botany2017
Cold acclimation alters DNA methylation patterns and confers tolerance to heat and increases growth rate in Brassica rapa.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 141 citations in OpenAlex.
- Transcriptome and DNA Methylation Analyses Provide Insight into Environmental Adaptation in Northern and Southern Populations ofAnimals : an open access journal from MDPI · 2026Article
- Canalized to heat, plastic to cold: adaptive coordination of leaf and seed strategies in populations spanning an elevational gradient.The New phytologist · 2026Article
- Differences in leaf heat and drought tolerance but not cold tolerance between karst and non-karst forest plants.Plant diversity · 2026Article
- New insights into controlling horticultural crop quality deterioration caused by cold stress: epigenetic modification.Horticulture research · 2026Article
- Epigenetic modifications in plant abiotic stress adaptation: towards climate-resilient and sustainable crop improvement.Frontiers in plant science · 2026Review
- DNA methylation contributes to plant acclimation to naturally fluctuating light.The New phytologist · 2025Article
- Integrated analysis of transcriptomic and metabolomic reveal insights into low-temperature stress in sainfoin (Onobrychis viciifolia) seedlings.BMC plant biology · 2025Article
- Review
- Article
- Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage.BMC plant biology · 2025Article
- DNA methylation confers epigenetic changes in cold-adapted microorganisms in response to cold stress.Extremophiles : life under extreme conditions · 2025Article
- Epigenetic odyssey to decrypt the hidden code for sustainable brassica production: enhancing yield, stress resilience and nutritional quality.Frontiers in plant science · 2025Review
- Genome-wide DNA methylation and their transgenerational pattern differ in Arabidopsis thaliana populations originated along the elevation of West Himalaya.BMC plant biology · 2024Article
- Impact of Abiotic Stress on Rice and the Role of DNA Methylation in Stress Response Mechanisms.Plants (Basel, Switzerland) · 2024Review
- Molecular Mechanisms Underlying Freezing Tolerance in Plants: Implications for Cryopreservation.International journal of molecular sciences · 2024Review
- Unravelling the Epigenetic Code: DNA Methylation in Plants and Its Role in Stress Response.Epigenomes · 2024Review
- Methyl-Sensitive Amplification Polymorphism (MSAP) Analysis Provides Insights into the DNA Methylation Changes Underlying Adaptation to Low Temperature ofPlants (Basel, Switzerland) · 2024Article
- Recent Advances in Studies of Genomic DNA Methylation and Its Involvement in Regulating Drought Stress Response in Crops.Plants (Basel, Switzerland) · 2024Review
- Systematic Analysis of DNA Demethylase Gene Families in Foxtail Millet (International journal of molecular sciences · 2024Article
- Increased DNA methylation contributes to the early ripening of pear fruits during domestication and improvement.Genome biology · 2024Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epigenetic modifications are implicated in plant adaptations to abiotic stresses. Exposure of plants to one stress can induce resistance to other stresses, a process termed cross-adaptation, which is not well understood. In this study, we aimed to unravel the epigenetic basis of elevated heat-tolerance in cold-acclimated Brassica rapa by conducting a genome-wide DNA methylation analysis of leaves from control (CK) and cold-acclimated (CA) plants. We found that both methylation and demethylation occurred during cold acclimation. Two significantly altered pathways, malate dehydrogenase activity and carbon fixation, and 1562 differentially methylated genes, including BramMDH1, BraKAT2, BraSHM4, and Bra4CL2, were identified in CA plants. Genetic validation and treatment of B. rapa with 5-aza-2-deoxycytidine (Aza) suggested that promoter demethylation of four candidate genes increased their transcriptional activities. Physiological analysis suggested that elevated heat-tolerance and high growth rate were closely related to increases in organic acids and photosynthesis, respectively. Functional analyses demonstrated that the candidate gene BramMDH1 (mMDH: mitochondrial malate dehydrogenase) directly enhances organic acids and photosynthesis to increase heat-tolerance and growth rate in Arabidopsis. However, Aza-treated B. rapa, which also has elevated BramMDH1 levels, did not exhibit enhanced heat-tolerance. We therefore suggest that DNA demethylation alone is not sufficient to increase heat-tolerance. This study demonstrates that altered DNA methylation contributes to cross-adaptation.
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