ArticleThe New phytologist2018
AtSERPIN1 is an inhibitor of the metacaspase AtMC1-mediated cell death and autocatalytic processing in planta.
Article in The New phytologist, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 58 citations in OpenAlex.
- Identification of genes controlling grain size in barley through RNA-seq and weighted gene co-expression network analysis.Functional & integrative genomics · 2026Article
- Plant caspase-like proteins: from function identification to application in winter rapeseed genetic breeding.Frontiers in plant science · 2026Review
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- Transcriptomic analysis of reduced thermotolerance inFrontiers in plant science · 2026Article
- Silenced cutters: mechanisms and effects of protease inhibition in plant-pathogen interactions.Journal of experimental botany · 2025Review
- The receptor MIK2 interacts with the kinase RKS1 to control quantitative disease resistance to Xanthomonas campestris.Plant physiology · 2024Article
- RNA silencing is a key regulatory mechanism in the biocontrol fungus Clonostachys rosea-wheat interactions.BMC biology · 2024Article
- TcSERPIN, an inhibitor that interacts with cocoa defense proteins and has biotechnological potential against human pathogens.Frontiers in plant science · 2024Article
- Plant Serpins: Potential Inhibitors of Serine and Cysteine Proteases with Multiple Functions.Plants (Basel, Switzerland) · 2023Review
- Arabidopsis metacaspase MC1 localizes in stress granules, clears protein aggregates, and delays senescence.The Plant cell · 2023Article
- The boundary of life and death: changes in mitochondrial and cytosolic proteomes associated with programmed cell death ofFrontiers in plant science · 2023Article
- Detection and Quantification of the Hypersensitive Response Cell Death in Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Comprehensive Phylogenetic Analysis of the Serpin Superfamily.Molecular biology and evolution · 2021Article
- Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus.Plant & cell physiology · 2021Article
- Cell Death in Plant Immunity.Cold Spring Harbor perspectives in biology · 2020Review
- Biochar Administration to San Marzano Tomato Plants Cultivated Under Low-Input Farming Increases Growth, Fruit Yield, and Affects Gene Expression.Frontiers in plant science · 2020Article
- Article
- Serpins: Genome-Wide Characterisation and Expression Analysis of the Serine Protease Inhibitor Family inG3 (Bethesda, Md.) · 2019Article
- Plant proteases during developmental programmed cell death.Journal of experimental botany · 2019Review
- Noncanonical interactions between serpin and β-amylase in barley grain improve β-amylase activityPlant direct · 2018Article
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9 authors at 5 institutions in 4 countries.
Funding
Abstract
The hypersensitive response (HR) is a localized programmed cell death phenomenon that occurs in response to pathogen recognition at the site of attempted invasion. Despite more than a century of research on HR, little is known about how it is so tightly regulated and how it can be contained spatially to a few cells. AtMC1 is an Arabidopsis thaliana plant metacaspase that positively regulates the HR. Here, we used an unbiased approach to identify new AtMC1 regulators. Immunoaffinity purification of AtMC1-containing complexes led us to the identification of the protease inhibitor AtSerpin1. Our data clearly showed that coimmunoprecipitation between AtMC1 and AtSerpin1 and formation of a complex between them was lost upon mutation of the AtMC1 catalytic site, and that the AtMC1 prodomain was not required for the interaction. AtSerpin1 blocked AtMC1 self-processing and inhibited AtMC1-mediated cell death. Our results constitute an in vivo example of a Serpin acting as a suicide inhibitor in plants, reminiscent of the activity of animal or viral serpins on immune/cell death regulators, including caspase-1. These results indicate a conserved function of a protease inhibitor on cell death regulators from different kingdoms with unrelated modes of action (i.e. caspases vs metacaspases).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.