ArticleScientific reports2017
Boreal pollen contain ice-nucleating as well as ice-binding 'antifreeze' polysaccharides.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Biochemical characterization of a fungal ice nucleation protein from Podila clonocystis.Scientific reports · 2026Article
- A previously unrecognized class of fungal ice-nucleating proteins with bacterial ancestry.Science advances · 2026Article
- Polysaccharides─Important Constituents of Ice-Nucleating Particles of Marine Origin.Environmental science & technology · 2025Article
- Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges.Biomicrofluidics · 2025Review
- Evaluation of Antifreeze Activity of Enzyme-Treated Extract from Mushroom Cell Wall.Journal of applied glycoscience · 2025Article
- High-speed cryo-microscopy reveals that ice-nucleating proteins ofScience advances · 2024Article
- Measurement of Ice Nucleation Activity of Biological Samples.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Cryopreservation of assay-ready hepatocyte monolayers by chemically-induced ice nucleation: preservation of hepatic function and hepatotoxicity screening capabilities.Biomaterials science · 2023Article
- Functional aggregation of cell-free proteins enables fungal ice nucleation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation.Chemical communications (Cambridge, England) · 2023Article
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- A highly active mineral-based ice nucleating agent supportsJournal of the Royal Society, Interface · 2023Article
- Lichen species across Alaska produce highly active and stable ice nucleators.Biogeosciences (Online) · 2023Article
- Poly(vinyl alcohol) Molecular Bottlebrushes Nucleate Ice.Biomacromolecules · 2022Article
- Synthesis of poly(vinyl alcohol) by blue light bismuth oxide photocatalysed RAFT. Evaluation of the impact of freeze/thaw cycling on ice recrystallisation inhibition.Polymer chemistry · 2022Article
- Pollen derived macromolecules serve as a new class of ice-nucleating cryoprotectants.Scientific reports · 2022Article
- The role of structural order in heterogeneous ice nucleation.Chemical science · 2022Article
- Polymer Self-Assembly Induced Enhancement of Ice Recrystallization Inhibition.Journal of the American Chemical Society · 2021Article
- The contribution of black carbon to global ice nucleating particle concentrations relevant to mixed-phase clouds.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ice nucleation and growth is an important and widespread environmental process. Accordingly, nature has developed means to either promote or inhibit ice crystal formation, for example ice-nucleating proteins in bacteria or ice-binding antifreeze proteins in polar fish. Recently, it was found that birch pollen release ice-nucleating macromolecules when suspended in water. Here we show that birch pollen washing water exhibits also ice-binding properties such as ice shaping and ice recrystallization inhibition, similar to antifreeze proteins. We present spectroscopic evidence that both the ice-nucleating as well as the ice-binding molecules are polysaccharides bearing carboxylate groups. The spectra suggest that both polysaccharides consist of very similar chemical moieties, but centrifugal filtration indicates differences in molecular size: ice nucleation occurs only in the supernatant of a 100 kDa filter, while ice shaping is strongly enhanced in the filtrate. This finding may suggest that the larger ice-nucleating polysaccharides consist of clusters of the smaller ice-binding polysaccharides, or that the latter are fragments of the ice-nucleating polysaccharides. Finally, similar polysaccharides released from pine and alder pollen also display both ice-nucleating as well as ice-binding ability, suggesting a common mechanism of interaction with ice among several boreal pollen with implications for atmospheric processes and antifreeze protection.
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