ArticleBMC genomics2016
Functional analysis for gut microbes of the brown tree frog (Polypedates megacephalus) in artificial hibernation.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 73 citations in OpenAlex.
- Is the Impact of Starvation on the Gut Microbiota Specific or Unspecific to Anorexia Nervosa? A Narrative Review Based on a Systematic Literature Search.Current neuropharmacology · 2018Pooled it
- Stochasticity Prevails but Differs: Tissue-Specific Assembly of Gut Microbiomes Across Seasons in an Amphibian Model.Ecology and evolution · 2026Article
- Responses of host energy status, intestinal structure and gut microbiota during post-hibernation recovery in high- and low-altitude populations of the plateau frogFrontiers in physiology · 2026Article
- The Immune-Antioxidant Trade-Off Mediated by Actinobacteria Drives Niche Differentiation: Physiological and Gut Microbiota Responses of Two Cold-Adapted Brown Frog Species to Contrasting Peak Daily Habitat Temperatures.Animals : an open access journal from MDPI · 2025Article
- Comparative Analysis of Environmental and Host-Associated Microbiome inMicroorganisms · 2025Article
- Impact of Light Spectrum on Tadpole Physiology and Gut Microbiota in the Dybowski's Frog (Animals : an open access journal from MDPI · 2025Article
- Diversity and Composition of Gut Microbiota in Different Developmental Stages of the Tibetan Toad (Animals : an open access journal from MDPI · 2025Article
- Comparative gut microbiome research through the lens of ecology: theoretical considerations and best practices.Biological reviews of the Cambridge Philosophical Society · 2025Review
- Season, Body Condition and Developmental Stage Influence the Gut Microbiota of the Sole Living Rhynchocephalian Reptile (Ecology and evolution · 2025Article
- Research Status and Prospect of Amphibian Symbiotic Microbiota.Animals : an open access journal from MDPI · 2025Review
- Culture-Independent Species-Level Taxonomic and Functional Characterisation of Bacteroides, the Core Bacterial Genus Within Reptile Guts.Molecular ecology · 2025Article
- Dynamics in gut microbiota diversity, composition, and assembly reveal the adaptability of invasive snailFrontiers in microbiology · 2025Article
- From Skin to Gut: Understanding Microbial Diversity in Rana amurensis and R. dybowskii.Current microbiology · 2024Article
- The Circadian Rhythm of the Behavior and Gut Microbiota in Dybowski's Frogs (Life (Basel, Switzerland) · 2024Article
- Gut microbiome diversity and function during hibernation and spring emergence in an aquatic frog.PloS one · 2024Article
- Microbial communities are thermally more sensitive in warm-climate lizards compared with their cold-climate counterparts.Frontiers in microbiology · 2024Article
- The structural discrepancy between the small and large gut microbiota of Asiatic toad (Bufo gargarizans) during hibernation.Folia microbiologica · 2023Article
- The succession of gut microbiota in the concave-eared torrent frog (Ecology and evolution · 2023Article
- Characteristics and a comparison of the gut microbiota in two frog species at the beginning and end of hibernation.Frontiers in microbiology · 2023Article
- Dynamic changes of fecal microbiota in a weight-change model of Bama minipigs.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAnnual hibernation is an adaptation that helps many animals conserve energy during food shortage in winter. This natural cycle is also accompanied by a remodeling of the intestinal immune system, which is an aspect of host biology that is both influenced by, and can itself influence, the microbiota. In amphibians, the bacteria in the intestinal tract show a drop in bacterial counts. The proportion of pathogenic bacteria is greater in hibernating frogs than that found in nonhibernating frogs. This suggests that some intestinal gut microbes in amphibians can be maintained and may contribute to the functions in this closed ecosystem during hibernation. However, these results were derived from culture-based approaches that only covered a small portion of bacteria in the intestinal tract.
methodsIn this study, we use a more comprehensive analysis, including bacterial appearance and functional prediction, to reveal the global changes in gut microbiota during artificial hibernation via high-throughput sequencing technology.
resultsOur results suggest that artificial hibernation in the brown tree frog (Polypedates megacephalus) could reduce microbial diversity, and artificially hibernating frogs tend to harbor core operational taxonomic units that are rarely distributed among nonhibernating frogs. In addition, artificial hibernation increased significantly the relative abundance of the red-leg syndrome-related pathogenic genus Citrobacter. Furthermore, functional predictions via PICRUSt and Tax4Fun suggested that artificial hibernation has effects on metabolism, disease, signal transduction, bacterial infection, and primary immunodeficiency.
conclusionsWe infer that artificial hibernation may impose potential effects on primary immunodeficiency and increase the risk of bacterial infections in the brown tree frog.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.