ArticlePlant cell reports2017
Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere.
Article in Plant cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Read-mapping signatures for molecular characterization of transgenic DNA insertions in paired-end Illumina data.BMC biotechnology · 2026Article
- T-DNAreader: fast and precise identification of T-DNA insertion sites in plant genomes using RNA sequencing data.Genome biology · 2025Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- T-DNA characterization of genetically modified 3-R-gene late blight-resistant potato events with a novel procedure utilizing the Samplix XdropFrontiers in plant science · 2024Article
- Characterization of integration sites and transfer DNA structures in Agrobacterium-mediated transgenic events of maize inbred B104.G3 (Bethesda, Md.) · 2023Article
- Foreign DNA detection in genome-edited potatoes by high-throughput sequencing.Scientific reports · 2023Article
- A modified Agrobacterium-mediated transformation for two oomycete pathogens.PLoS pathogens · 2023Article
- Identifying pathogenicity-related genes in the pathogenFrontiers in microbiology · 2023Article
- The translocated virulence protein VirD5 causes DNA damage and mutation duringScience advances · 2022Article
- Unintended Genomic Outcomes in Current and Next Generation GM Techniques: A Systematic Review.Plants (Basel, Switzerland) · 2022Review
- FFGA1 Protein Is Essential for Regulating Vegetative Growth, Cell Wall Integrity, and Protection against Stress inJournal of fungi (Basel, Switzerland) · 2022Article
- Exploring Genomic Variations in Nematode-Resistant Mutant Rice Lines.Frontiers in plant science · 2022Article
- Identification and Validation of Genetic Variations in Transgenic Chinese Cabbage Plants (Genes · 2021Article
- Article
- No Evidence of Unexpected Transgenic Insertions in T1190 - A Transgenic Apple Used in Rapid Cycle Breeding - Following Whole Genome Sequencing.Frontiers in plant science · 2021Article
- Strategies to produce T-DNA free CRISPRed fruit trees via Agrobacterium tumefaciens stable gene transfer.Scientific reports · 2020Article
- Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.Transgenic research · 2020Article
- Rapid and Detailed Characterization of Transgene Insertion Sites in Genetically Modified Plants via Nanopore Sequencing.Frontiers in plant science · 2020Article
- Generation ofMicroorganisms · 2019Article
- Mutagenesis in Rice: The Basis for Breeding a New Super Plant.Frontiers in plant science · 2019Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageTransformation resulted in deletions and translocations at T-DNA inserts, but not in genome-wide small mutations. A tiny T-DNA splinter was detected that probably would remain undetected by conventional techniques. We investigated to which extent Agrobacterium tumefaciens-mediated transformation is mutagenic, on top of inserting T-DNA. To prevent mutations due to in vitro propagation, we applied floral dip transformation of Arabidopsis thaliana. We re-sequenced the genomes of five primary transformants, and compared these to genomic sequences derived from a pool of four wild-type plants. By genome-wide comparisons, we identified ten small mutations in the genomes of the five transgenic plants, not correlated to the positions or number of T-DNA inserts. This mutation frequency is within the range of spontaneous mutations occurring during seed propagation in A. thaliana, as determined earlier. In addition, we detected small as well as large deletions specifically at the T-DNA insert sites. Furthermore, we detected partial T-DNA inserts, one of these a tiny 50-bp fragment originating from a central part of the T-DNA construct used, inserted into the plant genome without flanking other T-DNA. Because of its small size, we named this fragment a T-DNA splinter. As far as we know this is the first report of such a small T-DNA fragment insert in absence of any T-DNA border sequence. Finally, we found evidence for translocations from other chromosomes, flanking T-DNA inserts. In this study, we showed that next-generation sequencing (NGS) is a highly sensitive approach to detect T-DNA inserts in transgenic plants.
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