Evidence map›Paper›PMID 28154867›Full record

ArticleLab on a chip2017

Single cell-laden protease-sensitive microniches for long-term culture in 3D.

Philipp S Lienemann, Torsten Rossow, Angelo S Mao, Queralt Vallmajo-Martin, Martin Ehrbar, David J Mooney

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lab on a chip, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Single-Cell Microgels for Diagnostics and Therapeutics.Advanced functional materials · 2021
    Article
  13. Review
  14. Article
  15. Article
  16. Microfluidics-Based Single-Cell Research for Intercellular Interaction.Frontiers in cell and developmental biology · 2021
    Review
  17. Article
  18. Review
  19. Regenerative Therapies for Spinal Cord Injury.Tissue engineering. Part B, Reviews · 2019
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Philipp S LienemannJohn A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA. mooneyd@seas.harvard.edu and Wyss Institute for Biologically Inspired Engineering, Cambridge, MA 02138, USA.ORCID 0000-0002-0272-3988
Torsten RossowJohn A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA. mooneyd@seas.harvard.edu and Wyss Institute for Biologically Inspired Engineering, Cambridge, MA 02138, USA.
Angelo S MaoJohn A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA. mooneyd@seas.harvard.edu and Wyss Institute for Biologically Inspired Engineering, Cambridge, MA 02138, USA.
Queralt Vallmajo-MartinDepartment of Obstetrics, University Hospital Zurich, University of Zurich, Schmelzbergstr. 12, 8091 Zurich, Switzerland.
Martin EhrbarDepartment of Obstetrics, University Hospital Zurich, University of Zurich, Schmelzbergstr. 12, 8091 Zurich, Switzerland.
David J MooneyJohn A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA. mooneyd@seas.harvard.edu and Wyss Institute for Biologically Inspired Engineering, Cambridge, MA 02138, USA.

Funding

Building the Hematopoietic Stem Cell NicheR01EB014703 · NIBIB · HARVARD UNIVERSITY · PI MOONEY, DAVID J, SCADDEN, DAVID T · 2011 to 2014
$3.1M
NIBIB NIH HHS R01 EB014703
6 · The paper itself

Abstract

Single cell-laden three-dimensional (3D) microgels that can serve to mimic stem cell niches in vitro, and are therefore termed microniches, can be efficiently fabricated by droplet-based microfluidics. In this technique an aqueous polymer solution along with a highly diluted cell solution is injected into a microfluidic device to create monodisperse pre-microgel droplets that are then solidified by a polymer crosslinking reaction to obtain monodisperse single cell-laden microniches. However, problems limiting this approach studying the fate of single cells include Poisson encapsulation statistics that result in mostly empty microniches, and cells egressing from the microniches during subsequent cell culture. Here, we present a strategy to bypass Poisson encapsulation statistics in synthetic microniches by selective crosslinking of only cell-laden pre-microgel droplets. Furthermore, we show that we can position cells in the center of the microniches, and that even in protease-sensitive microniches this greatly reduces cell egress. Collectively, we present the development of a versatile protocol that allows for unprecedented efficiency in creation of synthetic protease-sensitive microniches for probing single stem cell fate in 3D.

Indexed as

AnimalsCell Culture TechniquesCell LineCellular MicroenvironmentMiceMicrofluidic Analytical TechniquesPeptide HydrolasesSingle-Cell AnalysisPeptide Hydrolases

Identifiers

PMID28154867
PMCPMC5481164

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.