ArticleTalanta2017
Optimizing 2D gas chromatography mass spectrometry for robust tissue, serum and urine metabolite profiling.
Article in Talanta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 63 citations in OpenAlex.
- Article
- Urine Recirculation During Normothermic Kidney Preservation Improves Energy Balance Involving the Urea and Citric Acid Cycles.Transplantation direct · 2026Article
- Nontargeted Screening in Environmental Monitoring Programs: An Update.Advances in experimental medicine and biology · 2026Review
- Separative Techniques Coupled to Mass Spectrometry for Metabolomic Analysis.Advances in experimental medicine and biology · 2026Review
- Article
- Delayed diagnosis in inflammatory bowel disease: Time to consider solutions.World journal of gastroenterology · 2024Article
- Association of Indoleamine 2,3-Dioxygenase (IDO) Activity with Outcome after Cardiac Surgery in Adult Patients.Metabolites · 2024Article
- Advances in Mass Spectrometry-Based Blood Metabolomics Profiling for Non-Cancer Diseases: A Comprehensive Review.Metabolites · 2024Review
- Metabolomics: A useful tool for ischemic stroke research.Journal of pharmaceutical analysis · 2023Review
- Biallelic FBXW7 knockout induces AKAP8-mediated DNA damage in neighbouring wildtype cells.Cell death discovery · 2023Article
- Unlocking the secrets of the microbiome: exploring the dynamic microbial interplay with humans through metabolomics and their manipulation for synthetic biology applications.The Biochemical journal · 2023Review
- CRISP: a deep learning architecture for GC × GC-TOFMS contour ROI identification, simulation and analysis in imaging metabolomics.Briefings in bioinformatics · 2022Article
- Article
- Modifying Chromatography Conditions for Improved Unknown Feature Identification in Untargeted Metabolomics.Analytical chemistry · 2021Article
- Putative markers for the detection of early-stage bladder cancer selected by urine metabolomics.BMC bioinformatics · 2021Article
- Dual role of ER stress in response to metabolic co-targeting and radiosensitivity in head and neck cancer cells.Cellular and molecular life sciences : CMLS · 2021Article
- Comprehensive Two-Dimensional Gas Chromatography Mass Spectrometry-Based Metabolomics.Advances in experimental medicine and biology · 2021Article
- Article
- Article
- Glutaminolysis and Glycolysis Are Essential for Optimal Replication of Marek's Disease Virus.Journal of virology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-dimensional gas chromatography mass spectrometry (GCxGC-MS) is utilized to an increasing extent in biomedical metabolomics. Here, we established and adapted metabolite extraction and derivatization protocols for cell/tissue biopsy, serum and urine samples according to their individual properties. GCxGC-MS analysis revealed detection of ~600 molecular features from which 165 were characterized representing different classes such as amino acids, fatty acids, lipids, carbohydrates, nucleotides and small polar components of glycolysis and the Krebs cycle using electron impact (EI) spectrum matching and validation using external standard compounds. Advantages of two-dimensional gas chromatography based resolution were demonstrated by optimizing gradient length and separation through modulation between the first and second column, leading to a marked increase in metabolite identification due to improved separation as exemplified for lactate versus pyruvate, talopyranose versus methyl palmitate and inosine versus docosahexaenoic acid. Our results demonstrate that GCxGC-MS represents a robust metabolomics platform for discovery and targeted studies that can be used with samples derived from the clinic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.