Evidence map›Paper›PMID 28152603›Full record

ArticleJournal of proteome research2017

Large-Scale and Targeted Quantitative Cross-Linking MS Using Isotope-Labeled Protein Interaction Reporter (PIR) Cross-Linkers.

Xuefei Zhong, Arti T Navare, Juan D Chavez, Jimmy K Eng, Devin K Schweppe, James E Bruce

Abstract read
In one paragraph

Article in Journal of proteome research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
4.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 42 citations in OpenAlex.

  1. Kinetic principles of chemical cross-link formation for protein-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2024
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  10. Crosslinking mass spectrometry: A link between structural biology and systems biology.Protein science : a publication of the Protein Society · 2021
    Review
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  17. Probing HMolecular & cellular proteomics : MCP · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Xuefei ZhongDepartment of Genome Sciences, University of Washington , Seattle, Washington 98109, United States.
Arti T NavareDepartment of Genome Sciences, University of Washington , Seattle, Washington 98109, United States.
Juan D ChavezDepartment of Genome Sciences, University of Washington , Seattle, Washington 98109, United States.
Jimmy K EngDepartment of Genome Sciences, University of Washington , Seattle, Washington 98109, United States.
Devin K SchweppeDepartment of Genome Sciences, University of Washington , Seattle, Washington 98109, United States.
James E BruceDepartment of Genome Sciences, University of Washington , Seattle, Washington 98109, United States.ORCID 0000-0001-6441-6089
University of Washington · US

Funding

Protein Interaction Technology CoreU19AI107775 · NIAID · UNIVERSITY OF WASHINGTON · PI BRITTNACHER, MITCHELL J · 2013 to 2017
$7.7M
Quantitation of protein interactions in cancer cellsR01GM086688 · NIGMS · UNIVERSITY OF WASHINGTON · PI BRUCE, JAMES EDWARD · 2009 to 2019
$4.2M
An LC-FTICR Mass Spectrometer for Proteomics and Metabolomics ResearchS10RR025107 · NCRR · UNIVERSITY OF WASHINGTON · PI BRUCE, JAMES EDWARD · 2008 to 2008
$990k
NCRR NIH HHS S10 RR025107NIAID NIH HHS U19 AI107775NIGMS NIH HHS R01 GM086688
6 · The paper itself

Abstract

Quantitative measurement of chemically cross-linked proteins is crucial to reveal dynamic information about protein structures and protein-protein interactions and how these are differentially regulated during stress, aging, drug treatment, and most perturbations. Previously, we demonstrated how quantitative in vivo cross-linking (CL) with stable isotope labeling of amino acids in cell culture (SILAC) enables both heritable and dynamic changes in cells to be visualized. In this work, we demonstrate the technical feasibility of proteome-scale quantitative in vivo CL-MS using isotope-labeled protein interaction reporter (PIR) cross-linkers and E. coli as a model system. This isotope-labeled cross-linkers approach, combined with Real-time Analysis of Cross-linked peptide Technology (ReACT) previously developed in our lab, enables the quantification of 941 nonredundant cross-linked peptide pairs from a total of 1213 fully identified peptide pairs in two biological replicate samples through comparison of MS

Indexed as

ProteomicsAmino AcidsCross-Linking ReagentsEscherichia coliIsotope LabelingPeptidesProteomeAmino AcidsCross-Linking ReagentsPeptidesProteomein vivo protein interactionsisotope labelingPIRproteome-scalequantitative CL−MS

Identifiers

PMID28152603
PMCPMC5513479
OpenAlexW2552262422

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.