ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2017
Quantifying three-dimensional morphology and RNA from individual embryos.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Parallel sensory compensation following independent subterranean colonization by groundwater salamanders (Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Quantifying the relationship between cell proliferation and morphology during development of the face.Development (Cambridge, England) · 2025Article
- Buffered Lugol's Iodine Preserves DNA Fragment Lengths.Integrative organismal biology (Oxford, England) · 2024Article
- Phosphotungstic acid-enhanced microCT: Optimized protocols for embryonic and early postnatal mice.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Nonlinear gene expression-phenotype relationships contribute to variation and clefting in the A/WySn mouse.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Quantitative analysis of morphogenesis aids our understanding of developmental processes by providing a method to link changes in shape with cellular and molecular processes. Over the last decade, many methods have been developed for 3D imaging of embryos using microCT scanning to quantify the shape of embryos during development. These methods generally involve a powerful, cross-linking fixative such as paraformaldehyde to limit shrinkage during the CT scan. However, the extended time frames that these embryos are incubated in such fixatives prevent use of the tissues for molecular analysis after microCT scanning. This is a significant problem because it limits the ability to correlate variation in molecular data with morphology at the level of individual embryos. Here we outline a novel method that allows RNA, DNA, or protein isolation following CT scan while also allowing imaging of different tissue layers within the developing embryo. We show shape differences early in craniofacial development (E11.5) between common mouse genetic backgrounds, and demonstrate that we are able to generate RNA from these embryos after CT scanning that is suitable for downstream real time PCR (RT-PCR) and RNAseq analyses. Developmental Dynamics 246:431-436, 2017. © 2017 Wiley Periodicals, Inc.
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Registered trials
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