ArticlePloS one2017
Dengue virus serotype 2 infection alters midgut and carcass gene expression in the Asian tiger mosquito, Aedes albopictus.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Bloodmeals fuel dengue virus replication in the female mosquitoJournal of virology · 2024Article
- Biased virus transmission following sequential coinfection of Aedes aegypti with dengue and Zika viruses.PLoS neglected tropical diseases · 2024Article
- Neofunctionalization driven by positive selection led to the retention of the loqs2 gene encoding an Aedes specific dsRNA binding protein.BMC biology · 2024Article
- Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis.Scientific reports · 2023Article
- Revised Annotation and Characterization of NovelBiology · 2022Article
- Isobaric tags for relative and absolute quantification-based proteomic analysis of host-pathogen protein interactions in the midgut ofFrontiers in microbiology · 2022Article
- Uncovering the Worldwide Diversity and Evolution of the Virome of the MosquitoesMicroorganisms · 2021Article
- Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection.PLoS neglected tropical diseases · 2021Article
- Vector competence of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquitoes for Mayaro virus.PLoS neglected tropical diseases · 2020Article
- Vector competence of Aedes aegypti and Culex quinquefasciatus from the metropolitan area of Guadalajara, Jalisco, Mexico for Zika virus.Scientific reports · 2019Article
- Differentiation of Long Non-Coding RNA and mRNA Expression Profiles in Male and FemaleFrontiers in genetics · 2019Article
- Understanding Oxidative Stress inViruses · 2018Article
- Characterization of dengue virus inMolecular biology research communications · 2018Article
- piRNA Profiling of Dengue Virus Type 2-Infected Asian Tiger Mosquito and Midgut Tissues.Viruses · 2018Article
- Correction: Dengue virus serotype 2 infection alters midgut and carcass gene expression in the Asian tiger mosquito, Aedes albopictus.PloS one · 2018Article
- Article
- RNA-Seq Comparison of Larval and Adult Malpighian Tubules of the Yellow Fever MosquitoFrontiers in physiology · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe Asian tiger mosquito, Aedes albopictus is currently an important vector for dengue, chikungunya and Zika virus, and its role in transmission of arthropod-borne viruses (arboviruses) may increase in the future due to its ability to colonize temperate regions. In contrast to Aedes aegypti, the dominant vector of dengue, chikungunya and Zika virus, genetic responses of Ae. albopictus upon infection with an arbovirus are not well characterized. Here we present a study of the changes in transcript expression in Ae. albopictus exposed to dengue virus serotype 2 via feeding on an artificial bloodmeal. METHODOLOGY/PRINCIPAL
findingsWe isolated midguts and midgut-free carcasses of Ae. albopictus fed on bloodmeals containing dengue virus as well as controls fed on virus-free control meals at day 1 and day 5 post-feeding. We confirmed infection of midguts from mosquitoes sampled on day 5 post-feeding via RT-PCR. RNAseq analysis revealed dynamic modulation of the expression of several putative immunity and dengue virus-responsive genes, some of whose expression was verified by qRT-PCR. For example, a serine protease gene was up-regulated in the midgut at 1 day post infection, which may potentially enhance mosquito susceptibility to dengue infection, while 14 leucine-rich repeat genes, previously shown to be involved in mosquito antiviral defenses, were down-regulated in the carcass at 5 days post infection. The number of significantly modulated genes decreased over time in midguts and increased in carcasses. CONCLUSION/SIGNIFICANCE: Dengue virus exposure results in the modulation of genes in a time- and site-specific manner. Previous literature on the interaction between mosquitoes and mosquito-borne pathogens suggests that most of the changes that occurred in Ae. albopictus exposed to DENV would favor virus infection. Many genes identified in this study warrant further characterization to understand their role in viral manipulation of and antiviral response of Ae. albopictus.
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