ArticlePloS one2017
Adipose tissue macrophages impair preadipocyte differentiation in humans.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Review
- Clinical Translation of Fisetin for Age-Related Diseases: Current Evidence and Future Opportunities.Nutrients · 2026Review
- Galectin-3 inhibitor selvigaltin dosed therapeuticallyFrontiers in physiology · 2026Article
- Harnessing Adipose Ferroptosis: A Promising Novel Pathway for Obesity Treatment.Current obesity reports · 2025Review
- The Impact of Resident Adipose Tissue Macrophages on Adipocyte Homeostasis and Dedifferentiation.International journal of molecular sciences · 2024Article
- Regional primary preadipocyte characteristics in humans with obesity and type 2 diabetes mellitus.Heliyon · 2024Review
- Therapeutic targeting of white adipose tissue metabolic dysfunction in obesity: mechanisms and opportunities.MedComm · 2024Review
- Metabolic Disorders, the Microbiome as an Endocrine Organ, and Their Relations with Obesity: A Literature Review.Journal of personalized medicine · 2023Review
- Adipose tissue macrophages as potential targets for obesity and metabolic diseases.Frontiers in immunology · 2023Review
- Adipose tissue aging is regulated by an altered immune system.Frontiers in immunology · 2023Review
- Immune Cell Regulation of White Adipose Progenitor Cell Fate.Frontiers in endocrinology · 2022Review
- Roles of estrogens, estrogen-like compounds, and endocrine disruptors in adipocytes.Frontiers in endocrinology · 2022Review
- Emerging Roles of Adipose Tissue in the Pathogenesis of Psoriasis and Atopic Dermatitis in Obesity.JID innovations : skin science from molecules to population health · 2022Review
- TNFα Mediates Inflammation-Induced Effects onCells · 2021Article
- Relationship Between Coronary Atheroma, Epicardial Adipose Tissue Inflammation, and Adipocyte Differentiation Across the Human Myocardial Bridge.Journal of the American Heart Association · 2021Article
- Testosterone treatment is associated with reduced adipose tissue dysfunction and nonalcoholic fatty liver disease in obese hypogonadal men.Journal of endocrinological investigation · 2021Observational
- Cellular Heterogeneity in Adipose Tissues.Annual review of physiology · 2021Review
- Adipose Tissue Fibrosis: Mechanisms, Models, and Importance.International journal of molecular sciences · 2020Review
- Red Raspberry Polyphenols Attenuate High-Fat Diet-Driven Activation of NLRP3 Inflammasome and its Paracrine Suppression of Adipogenesis via Histone Modifications.Molecular nutrition & food research · 2020Article
- The Effect of Early Rounds of ex vivo Expansion and Cryopreservation on the Adipogenic Differentiation Capacity of Adipose-Derived Stromal/Stem Cells.Scientific reports · 2019Article
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9 authors.
Funding
Abstract
aimThe physiologic mechanisms underlying the relationship between obesity and insulin resistance are not fully understood. Impaired adipocyte differentiation and localized inflammation characterize adipose tissue from obese, insulin-resistant humans. The directionality of this relationship is not known, however. The aim of the current study was to investigate whether adipose tissue inflammation is causally-related to impaired adipocyte differentiation.
methodsAbdominal subcutaneous(SAT) and visceral(VAT) adipose tissue was obtained from 20 human participants undergoing bariatric surgery. Preadipocytes were isolated, and cultured in the presence or absence of CD14+ macrophages obtained from the same adipose tissue sample. Adipocyte differentiation was quantified after 14 days via immunofluorescence, Oil-Red O, and adipogenic gene expression. Cytokine secretion by mature adipocytes cultured with or without CD14+macrophages was quantified.
resultsAdipocyte differentiation was significantly lower in VAT than SAT by all measures (p<0.001). With macrophage removal, SAT preadipocyte differentiation increased significantly as measured by immunofluorescence and gene expression, whereas VAT preadipocyte differentiation was unchanged. Adipocyte-secreted proinflammatory cytokines were higher and adiponectin lower in media from VAT vs SAT: macrophage removal reduced inflammatory cytokine and increased adiponectin secretion from both SAT and VAT adipocytes. Differentiation of preadipocytes from SAT but not VAT correlated inversely with systemic insulin resistance.
conclusionsThe current results reveal that proinflammatory immune cells in human SAT are causally-related to impaired preadipocyte differentiation, which in turn is associated with systemic insulin resistance. In VAT, preadipocyte differentiation is poor even in the absence of tissue macrophages, pointing to inherent differences in fat storage potential between the two depots.
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