ArticlePloS one2017
D-amino acids reduce Enterococcus faecalis biofilms in vitro and in the presence of antimicrobials used for root canal treatment.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 59 citations in OpenAlex.
- Antimicrobial efficacy of Odontopaste in endodontics: a systematic review.Evidence-based dentistry · 2024Pooled it
- Biological function of d-tryptophan: a bibliometric analysis and review.Frontiers in microbiology · 2024Pooled it
- O-Acetyl-L-homoserine sulfhydrylase from Thermotoga maritima has five enzyme activities.Extremophiles : life under extreme conditions · 2025Article
- The potential impact of iron supply on the development of starvedFrontiers in microbiology · 2025Article
- d-Methionine-induced DNases disperse establishedBiofilm · 2024Article
- D-tryptophan, an eco-friendly natural, safe, and healthy compound with antimicrobial activity against food-borne pathogens: A systematic review.Food science & nutrition · 2024Review
- Effect of sodium hypochlorite gel on bacteria associated with periodontal disease.Clinical oral investigations · 2024Article
- The Potential Uses of Baobab Tree's Medicinal Effects in Dentistry: A Literature Review.Cureus · 2023Review
- Promising applications of D-amino acids in periprosthetic joint infection.Bone research · 2023Review
- Antibiofilm Activity of LL-37 Peptide and D-Amino Acids Associated with Antibiotics Used in Regenerative Endodontics on an Ex Vivo Multispecies Biofilm Model.Life (Basel, Switzerland) · 2022Article
- Targeting Persistent Biofilm Infections: Reconsidering the Topography of the Infection Site during Model Selection.Microorganisms · 2022Review
- D-Amino Acids as a Biomarker in Schizophrenia.Diseases (Basel, Switzerland) · 2022Review
- Combination Therapies for Biofilm Inhibition and Eradication: A Comparative Review of Laboratory and Preclinical Studies.Frontiers in cellular and infection microbiology · 2022Review
- Identification and biochemical characterization of threonine dehydratase from the hyperthermophile Thermotoga maritima.Amino acids · 2021Article
- Antibacterial Effect of Endodontic Disinfections on Enterococcus Faecalis in Dental Root Canals-An In-Vitro Model Study.Materials (Basel, Switzerland) · 2021Article
- Disruption of Enterococcus Faecalis biofilms using individual and plasma polymer encapsulated D-amino acids.Clinical oral investigations · 2021Article
- Antibacterial Effect of High-Purity Nisin Alone and in Combination with D-Amino Acids or Chlorhexidine in an Endodontic-Like Biofilm Model.Antibiotics (Basel, Switzerland) · 2021Article
- Inhibition ofAntibiotics (Basel, Switzerland) · 2020Article
- d-Amino Acids and Lactic Acid Bacteria.Microorganisms · 2019Review
- d-amino Acids in Health and Disease: A Focus on Cancer.Nutrients · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterococcus faecalis is the most frequent species present in post-treatment disease and plays a significant role in persistent periapical infections following root canal treatment. Its ability to persist in stressful environments is inter alia, due to its ability to form biofilms. The presence of certain D-amino acids (DAAs) has previously been shown to reduce formation of Bacillus subtilis biofilms. The aims of this investigation were to determine if DAAs disrupt biofilms in early and late growth stages for clinical E. faecalis strains and to test their efficacy in disrupting E. faecalis biofilms grown in sub-minimum inhibitory concentrations of commonly used endodontic biocides. From thirty-seven E. faecalis strains, the ten "best" biofilm producers were used to test the ability of a mixture containing D-leucine, D-methionine, D-tyrosine and D-tryptophan to reduce biofilm growth over a period of 24, 72 and 144 hours and when compared to their cognate L-Amino Acids (LAAs). We have previously shown that sub-MIC levels of tetracycline and sodium hypochlorite promotes biofilm growth in clinical strains of E. faecalis. DAAs were therefore tested for their effectiveness to reduce biofilm growth in the presence of sub-minimal concentrations of sodium hypochlorite (NaOCl-0.031%) and Odontocide™ (0.25% w/v), and in the presence of Odontopaste™ (0.25% w/v). DAAs significantly reduced biofilm formation for all strains tested in vitro, while DAAs significantly reduced biofilm formation compared to LAAs. The inhibitory effect of DAAs on biofilm formation was concentration dependent. DAAs were also shown to be effective in reducing E. faecalis biofilms in the presence of Odontopaste™ and sub-MIC levels of NaOCl and Odontocide™. The results suggest that the inclusion of DAAs into current endodontic procedures may reduce E. faecalis biofilms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.