ArticleScientific reports2017
Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
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- Mechanism of Forced-Copy-Choice RNA Recombination by Enteroviral RNA-Dependent RNA Polymerases.ACS bio & med chem Au · 2025Article
- Improved precision, sensitivity, and adaptability of ordered two-template relay cDNA library preparation for RNA sequencing.RNA (New York, N.Y.) · 2025Article
- Targeted barcoding of variable antibody domains and individual transcriptomes of the human B-cell repertoire using Link-Seq.PNAS nexus · 2025Article
- Improved precision, sensitivity, and adaptability of Ordered Two-Template Relay cDNA library preparation for RNA sequencing.bioRxiv : the preprint server for biology · 2024Article
- Clamping-mediated incorporation of single-stranded DNA with concomitant DNA synthesis by Taq polymerase involves nick-translation.Scientific reports · 2024Article
- Mechanism of protein-primed template-independent DNA synthesis by Abi polymerases.Nucleic acids research · 2022Article
- Low-bias ncRNA libraries using ordered two-template relay: Serial template jumping by a modified retroelement reverse transcriptase.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Primer extension coupled with fragment analysis for rapid and quantitative evaluation of 5.8S rRNA isoforms.PloS one · 2021Article
- Non-templated addition and template switching by Moloney murine leukemia virus (MMLV)-based reverse transcriptases co-occur and compete with each other.The Journal of biological chemistry · 2019Article
- Structural Elucidation of Bisulfite Adducts to Pseudouridine That Result in Deletion Signatures during Reverse Transcription of RNA.Journal of the American Chemical Society · 2019Article
- Properties and efficient scrap-and-build repairing of mechanically sheared 3' DNA ends.Communications biology · 2019Article
- Factors Affecting the Tailing of Blunt End DNA with Fluorescent Pyrimidine dNTPs.Molecular biotechnology · 2018Article
- Optimization of single strand DNA incorporation reaction by Moloney murine leukaemia virus reverse transcriptase.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Template-primer binding affinity and RNase H cleavage specificity contribute to the strand transfer efficiency of HIV-1 reverse transcriptase.The Journal of biological chemistry · 2018Article
- Article
- The Small Protein HemP Is a Transcriptional Activator for the Hemin Uptake Operon in Burkholderia multivorans ATCC 17616.Applied and environmental microbiology · 2017Article
- Compounds that enhance the tailing activity of Moloney murine leukemia virus reverse transcriptase.Scientific reports · 2017Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Moloney murine leukemia virus reverse transcriptase (MMLV-RT) is a widely used enzyme for cDNA synthesis. Here we show that MMLV-RT has a strong template-independent polymerase activity using blunt DNA ends as substrate that generates 3' overhangs of A, C, G, or T. Nucleotides were appended efficiently in the order A > G > T > C, and tail lengths varied from 4 to 5, 2 to 7, 2 to 4, and 2 to 3 for A, C, G, and T, respectively. The activity was so strong that nearly all our test DNA ends were appended with at least one A, C, G, or T. The N-tailing activity of MMLV-RT was enhanced in the presence of Mn
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