ArticleScientific reports2017
Development of a multilocus-based approach for sponge (phylum Porifera) identification: refinement and limitations.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 28 citations in OpenAlex.
- Primer-Less Species Identification Throughout Fungal (International journal of molecular sciences · 2026Article
- Ethanolic extracts from deep marine sponges: A new frontier in antibacterial discovery from the Jordanian Gulf of Aqaba.Biomedical reports · 2026Article
- Transcriptomic Insights Into the Immune Repertoire of an Antarctic Sponge.Ecology and evolution · 2025Article
- Identification and phylogenetic analysis of marine sponges in the Jordanian Gulf of Aqaba using DNA barcoding.Heliyon · 2025Article
- Article
- Diversity and structure of the deep-sea sponge microbiome in the equatorial Atlantic Ocean.Microbiology (Reading, England) · 2024Article
- Discovery and biosynthetic assessment of 'Microbial genomics · 2023Article
- Genome-centric view of the microbiome in a new deep-sea glass sponge speciesFrontiers in microbiology · 2023Article
- Characterizing the bacterial communities associated with Mediterranean sponges: a metataxonomic analysis.Frontiers in microbiology · 2023Article
- Low genetic diversity and predation threaten a rediscovered marine sponge.Scientific reports · 2022Article
- Factors affecting the isolation and diversity of marine sponge-associated bacteria.Applied microbiology and biotechnology · 2022Article
- Antimicrobial Activities of Marine Sponge-Associated Bacteria.Microorganisms · 2021Article
- Not That Close to Mommy: Horizontal Transmission Seeds the Microbiome Associated with the Marine SpongeMicroorganisms · 2020Article
- Uncovering the hidden marine sponge microbiome by applying a multi-primer approach.Scientific reports · 2019Article
- Article
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For sponges (phylum Porifera), there is no reliable molecular protocol available for species identification. To address this gap, we developed a multilocus-based Sponge Identification Protocol (SIP) validated by a sample of 37 sponge species belonging to 10 orders from South Australia. The universal barcode COI mtDNA, 28S rRNA gene (D3-D5), and the nuclear ITS1-5.8S-ITS2 region were evaluated for their suitability and capacity for sponge identification. The highest Bit Score was applied to infer the identity. The reliability of SIP was validated by phylogenetic analysis. The 28S rRNA gene and COI mtDNA performed better than the ITS region in classifying sponges at various taxonomic levels. A major limitation is that the databases are not well populated and possess low diversity, making it difficult to conduct the molecular identification protocol. The identification is also impacted by the accuracy of the morphological classification of the sponges whose sequences have been submitted to the database. Re-examination of the morphological identification further demonstrated and improved the reliability of sponge identification by SIP. Integrated with morphological identification, the multilocus-based SIP offers an improved protocol for more reliable and effective sponge identification, by coupling the accuracy of different DNA markers.
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