ArticleScientific reports2017
Distinct requirement of Runx complexes for TCRβ enhancer activation at distinct developmental stages.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 19 citations in OpenAlex.
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- Enhancers in T Cell development and malignant lesions.Cell death discovery · 2024Review
- LAG-3 and PD-1 synergize on CD8Cell · 2024Article
- Cutting Edge: LAG3 Dimerization Is Required for TCR/CD3 Interaction and Inhibition of Antitumor Immunity.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Review
- Differential regulation of transcription factor T-bet induction during NK cell development and T helper-1 cell differentiation.Immunity · 2022Article
- Characterization of transcripts emanating from enhancer Eβ of the murine TCRβ locus.FEBS open bio · 2021Article
- Regulation of T-cell Receptor Gene Expression by Three-Dimensional Locus Conformation and Enhancer Function.International journal of molecular sciences · 2020Review
- Resistance to PD1 blockade in the absence of metalloprotease-mediated LAG3 shedding.Science immunology · 2020Article
- The Roles of RUNX Family Proteins in Development of Immune Cells.Molecules and cells · 2020Review
- Constitutive CD8 expression drives innate CD8Life science alliance · 2020Article
- Collaboration of MYC and RUNX2 in lymphoma simulates T-cell receptor signaling and attenuates p53 pathway activity.Journal of cellular biochemistry · 2019Article
- Programming for T-lymphocyte fates: modularity and mechanisms.Genes & development · 2019Review
- Adaptive plasticity of IL-10Nature immunology · 2019Article
- Requirement for intron structures in activating theProceedings of the National Academy of Sciences of the United States of America · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A TCRβ enhancer, known as the Eβ enhancer, plays a critical role in V(D)J recombination and transcription of the Tcrb gene. However, the coordinated action of trans-acting factors in the activation of Eβ during T cell development remains uncharacterized. Here, we characterized the roles of Runx complexes in the regulation of the Eβ function. A single mutation at one of the two Runx binding motifs within the Eβ severely impaired Tcrb activation at the initiation phase in immature thymocytes. However, TCRβ expression level in mature thymocytes that developed under such a single Runx site mutation was similar to that of the control. In contrast, mutations at two Runx motifs eliminated Eβ activity, demonstrating that Runx complex binding is essential to initiate Eβ activation. In cells expressing Tcrb harboring rearranged V(D)J structure, Runx complexes are dispensable to maintain TCRβ expression, whereas Eβ itself is continuously required for TCRβ expression. These findings imply that Runx complexes are essential for Eβ activation at the initiation phase, but are not necessary for maintaining Eβ activity at later developmental stages. Collectively, our results indicate that the requirements of trans-acting factor for Eβ activity are differentially regulated, depending on the developmental stage and cellular activation status.
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