ArticleJournal of plant research2017
Molecular cloning and functional analysis of the drought tolerance gene MsHSP70 from alfalfa (Medicago sativa L.).
Article in Journal of plant research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Identification of HSP70 family and screening of drought resistance genes in sugar beet.BMC genomics · 2025Article
- Tandem mass tag (TMT)-based quantitative proteomics analysis reveals the different responses of contrasting alfalfa varieties to drought stress.BMC genomics · 2024Article
- QTL Mapping and a Transcriptome Integrative Analysis Uncover the Candidate Genes That Control the Cold Tolerance of Maize Introgression Lines at the Seedling Stage.International journal of molecular sciences · 2023Article
- Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response toFrontiers in genetics · 2023Article
- Genome-Wide Identification, Characterization, and Expression Analysis of Glutamate Receptor-like Gene (Plants (Basel, Switzerland) · 2022Article
- Identification of QTL and candidate genes associated with biomass yield and Feed Quality in response to water deficit in alfalfa (Frontiers in plant science · 2022Article
- Genome-wide analysis of Hsp70 and Hsp100 gene families in Ziziphus jujuba.Cell stress & chaperones · 2021Article
- Transcriptome sequencing reveals hotspot mutation regions and dwarfing mechanisms in wheat mutants induced by γ-ray irradiation and EMS.Journal of radiation research · 2020Article
- Functional Analysis of theFrontiers in plant science · 2020Article
- Knockdown of CaHSP60-6 confers enhanced sensitivity to heat stress in pepper (Capsicum annuum L.).Planta · 2019Article
- Heat Shock Proteins: Dynamic Biomolecules to Counter Plant Biotic and Abiotic Stresses.International journal of molecular sciences · 2019Review
- Molecular Cloning and Functional Identification of a Squalene Synthase Encoding Gene from Alfalfa (International journal of molecular sciences · 2019Article
- A Gene Regulatory Network Controlled by BpERF2 and BpMYB102 in Birch under Drought Conditions.International journal of molecular sciences · 2019Article
- Isolation and Functional Characterization ofInternational journal of molecular sciences · 2019Article
- Genome-Wide Identification, Classification and Expression Analysis of theInternational journal of molecular sciences · 2018Article
- Improving the Yield and Nutritional Quality of Forage Crops.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat shock proteins (HSPs) are a ubiquitously expressed class of protective proteins that play a key role in plant response to stressful conditions. This study aimed to characterize and investigate the function of an HSP gene in alfalfa (Medicago sativa). MsHSP70, which contains a 2028-bp open reading frame, was identified through homology cloning. MsHSP70 shares high sequence identity (94.47%) with HSP70 from Medicago truncatula. Expression analysis of MsHSP70 in alfalfa organs revealed a relatively higher expression level in aerial organs such as flowers, stems and leaves than in roots. MsHSP70 was induced by heat shock, abscisic acid (ABA) and hydrogen peroxide. Transgenic Arabidopsis seedlings overexpressing MsHSP70 were hyposensitive to polyethylene glycol (PEG) and ABA treatments, suggesting that exogenous expression of MsHSP70 enhanced Arabidopsis tolerance to these stresses. Examination of physiological indexes related to drought and ABA stress demonstrated that in comparison with non-transgenic plants, T3 transgenic Arabidopsis plants had an increased proline content, higher superoxide dismutase (SOD) activity, and decreased malondialdehyde (MDA) content. Furthermore, higher relative water content (RWC) was detected in transgenic plants compared with non-transgenic plants under drought stress. These findings clearly indicate that molecular manipulation of MsHSP70 in plants can have substantial effects on stress tolerance.
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