ReviewMolecular genetics and genomics : MGG2017
A practical guide to studying G-quadruplex structures using single-molecule FRET.
Review in Molecular genetics and genomics : MGG, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Accessibility of telomeric overhangs to stabilizing small-molecule ligands.Nucleic acids research · 2026Article
- Investigating Potential 5' UTR G-Quadruplexes Within NRF2 mRNA.Current issues in molecular biology · 2026Article
- Reusable Microfluidic Chambers for Single-Molecule Microscopy.ACS applied materials & interfaces · 2024Article
- Protection of the Telomeric Junction by the Shelterin Complex.Journal of the American Chemical Society · 2024Article
- Protection of the Telomeric Junction by the Shelterin Complex.bioRxiv : the preprint server for biology · 2024Article
- Mapping fast DNA polymerase exchange during replication.Nature communications · 2024Article
- Stalling of Transcription by Putative G-quadruplex Sequences and CRISPR-dCas9.bioRxiv : the preprint server for biology · 2024Article
- Structure, Topology, and Stability of Multiple G-quadruplexes in Long Telomeric Overhangs.Journal of molecular biology · 2024Review
- Detecting secondary structure formation with FRET-PAINT.The Analyst · 2023Article
- Inhibited complete folding of consecutive human telomeric G-quadruplexes.Nucleic acids research · 2023Article
- Unravelling How Single-Stranded DNA Binding Protein Coordinates DNA Metabolism Using Single-Molecule Approaches.International journal of molecular sciences · 2023Review
- Pressure Tuning Studies of Four-Stranded Nucleic Acid Structures.International journal of molecular sciences · 2023Review
- Shelterin reduces the accessibility of telomeric overhangs.Nucleic acids research · 2022Article
- Emerging accessibility patterns in long telomeric overhangs.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences.Nucleic acids research · 2022Article
- Fast interaction dynamics of G-quadruplex and RGG-rich peptides unveiled in zero-mode waveguides.Nucleic acids research · 2021Article
- Characterization of G-Quadruplexes Folding/Unfolding Dynamics and Interactions with Proteins from Single-Molecule Force Spectroscopy.Biomolecules · 2021Review
- Interrogating accessibility of telomeric sequences with FRET-PAINT: evidence for length-dependent telomere compaction.Nucleic acids research · 2021Article
- High-yield purification of exceptional-quality, single-molecule DNA substrates.Journal of biological methods · 2021Article
- Quantifying the impact of small molecule ligands on G-quadruplex stability against Bloom helicase.Nucleic acids research · 2019Article
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4 authors.
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Abstract
In this article, we summarize the knowledge and best practices learned from bulk and single-molecule measurements to address some of the frequently experienced difficulties in single-molecule Förster resonance energy transfer (smFRET) measurements on G-quadruplex (GQ) structures. The number of studies that use smFRET to investigate the structure, function, dynamics, and interactions of GQ structures has grown significantly in the last few years, with new applications already in sight. However, a number of challenges need to be overcome before reliable and reproducible smFRET data can be obtained in measurements that include GQ. The annealing and storage conditions, the location of fluorophores on the DNA construct, and the ionic conditions of the experiment are some of the factors that are of critical importance for the outcome of measurements, and many of these manifest themselves in unique ways in smFRET assays. By reviewing these aspects and providing a summary of best practices, we aim to provide a practical guide that will help in successfully designing and performing smFRET studies on GQ structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.