ArticlePLoS genetics2017
Genome-wide exonic small interference RNA-mediated gene silencing regulates sexual reproduction in the homothallic fungus Fusarium graminearum.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Tandem repeat-induced sexual silencing: A Rid-dependent RNAi mechanism for fungal genome defense via translational repression.Science advances · 2025Article
- Methylation of mycovirus DNA is mediated by the RNAi machinery in vegetative hyphae of Fusarium graminearum.Nucleic acids research · 2025Article
- Article
- Fungal small RNA: unveiling the breakthroughs and promising applications.Frontiers in fungal biology · 2025Review
- Harnessing RNA interference for the control of Fusarium species: A critical review.Molecular plant pathology · 2024Review
- RNAi-Based Approaches to Control Mycotoxin Producers: Challenges and Perspectives.Journal of fungi (Basel, Switzerland) · 2024Review
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- Article
- The RNAi Machinery in the FungusNon-coding RNA · 2024Article
- Advances in UnderstandingGenes · 2024Review
- Interference of Small RNAs inJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Transcriptional Divergence Underpinning Sexual Development in the Fungal Class Sordariomycetes.mBio · 2022Article
- FoQDE2-dependent milRNA promotes Fusarium oxysporum f. sp. cubense virulence by silencing a glycosyl hydrolase coding gene expression.PLoS pathogens · 2022Article
- Exploring the Effectiveness and Durability of Trans-Kingdom Silencing of Fungal Genes in the Vascular PathogenInternational journal of molecular sciences · 2022Article
- Computational Analysis of HTS Data and Its Application in Plant Pathology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mycovirus-encoded suppressors of RNA silencing: Possible allies or enemies in the use of RNAi to control fungal disease in crops.Frontiers in fungal biology · 2022Review
- FgMet3 and FgMet14 related to cysteine and methionine biosynthesis regulate vegetative growth, sexual reproduction, pathogenicity, and sensitivity to fungicides inFrontiers in plant science · 2022Article
- Comparative profiling of canonical and non-canonical small RNAs in the rice blast fungus,Frontiers in microbiology · 2022Article
- Role of Dicer-Dependent RNA Interference in Regulating Mycoparasitic Interactions.Microbiology spectrum · 2021Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Various ascomycete fungi possess sex-specific molecular mechanisms, such as repeat-induced point mutations, meiotic silencing by unpaired DNA, and unusual adenosine-to-inosine RNA editing, for genome defense or gene regulation. Using a combined analysis of functional genetics and deep sequencing of small noncoding RNA (sRNA), mRNA, and the degradome, we found that the sex-specifically induced exonic small interference RNA (ex-siRNA)-mediated RNA interference (RNAi) mechanism has an important role in fine-tuning the transcriptome during ascospore formation in the head blight fungus Fusarium graminearum. Approximately one-third of the total sRNAs were produced from the gene region, and sRNAs with an antisense direction or 5'-U were involved in post-transcriptional gene regulation by reducing the stability of the corresponding gene transcripts. Although both Dicers and Argonautes partially share their functions, the sex-specific RNAi pathway is primarily mediated by FgDicer1 and FgAgo2, while the constitutively expressed RNAi components FgDicer2 and FgAgo1 are responsible for hairpin-induced RNAi. Based on our results, we concluded that F. graminearum primarily utilizes ex-siRNA-mediated RNAi for ascosporogenesis but not for genome defenses and other developmental stages. Each fungal species appears to have evolved RNAi-based gene regulation for specific developmental stages or stress responses. This study provides new insights into the regulatory role of sRNAs in fungi and other lower eukaryotes.
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