ArticleMemorias do Instituto Oswaldo Cruz2017
Identification of circo-like virus-Brazil genomic sequences in raw sewage from the metropolitan area of São Paulo: evidence of circulation two and three years after the first detection.
Article in Memorias do Instituto Oswaldo Cruz, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 24 citations in OpenAlex.
- Novel Virus Identification through Metagenomics: A Systematic Review.Life (Basel, Switzerland) · 2022Review
- Entamoeba and Giardia parasites implicated as hosts of CRESS viruses.Nature communications · 2020Article
- Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity.Viruses · 2020Article
- Viral metagenomics revealed diverse CRESS-DNA virus genomes in faeces of forest musk deer.Virology journal · 2020Article
- Novel Circo-Like Virus Detected in a Croatian Bat Population.Microbiology resource announcements · 2019Article
- Evaluation of the serum virome in calves persistently infected with Pestivirus A, presenting or not presenting mucosal disease.Virus genes · 2018Article
- Identification and genomic characterization of a novel CRESS DNA virus from a calf with severe hemorrhagic enteritis in China.Virus research · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTwo novel viruses named circo-like virus-Brazil (CLV-BR) hs1 and hs2 were previously discovered in a Brazilian human fecal sample through metagenomics. CLV-BR hs1 and hs2 possess a small circular DNA genome encoding a replication initiator protein (Rep), and the two genomes exhibit 92% nucleotide identity with each other. Phylogenetic analysis based on the Rep protein showed that CLV-BRs do not cluster with circoviruses, nanoviruses, geminiviruses or cycloviruses.
objectiveThe aim of this study was to search for CLV-BR genomes in sewage and reclaimed water samples from the metropolitan area of São Paulo, Brazil, to verify whether the first detection of these viruses was an isolated finding.
methodsSewage and reclaimed water samples collected concomitantly during the years 2005-2006 were purified and concentrated using methodologies designed for the study of viruses. A total of 177 treated reclaimed water samples were grouped into five pools, as were 177 treated raw sewage samples. Nucleic acid extraction, polymerase chain reaction (PCR) amplification and Sanger sequencing were then performed.e. FINDINGS CLV-BR: genomes were detected in two pools of sewage samples, p6 and p9. Approximately 28% and 51% of the CLV-BR genome was amplified from p6 and p9, respectively, including 76% of the Rep gene. The detected genomes are most likely related to CLV-BR hs1. Comparative analysis showed several synonymous substitutions within Rep-encoding sequences, suggesting purifying selection for this gene, as has been observed for other eukaryotic circular Rep-encoding single-stranded DNA (CRESS-DNA) viruses. MAIN
conclusionThe results therefore indicated that CLV-BR has continued to circulate in Brazil two and three years after first being detected.
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