Evidence map›Paper›PMID 28143904›Full record

ArticleScience signaling2017

AMPK promotes mitochondrial biogenesis and function by phosphorylating the epigenetic factors DNMT1, RBBP7, and HAT1.

Traci L Marin, Brendan Gongol, Fan Zhang, Marcy Martin, David A Johnson, Han Xiao, Yinsheng Wang, Shankar Subramaniam, Shu Chien, John Y-J Shyy

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science signaling, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
140citing papers in PubMed, 1 pooled it
8.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

140 citing papers in PubMed, 1 synthesis or guideline pooled it, 242 citations in OpenAlex.

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80 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Traci L MarinDepartment of Biochemistry, University of California, Riverside, Riverside, CA 92521, USA.
Brendan GongolDepartment of Biochemistry, University of California, Riverside, Riverside, CA 92521, USA.
Fan ZhangDepartment of Chemistry, University of California, Riverside, Riverside, CA 92521, USA.
Marcy MartinDepartment of Biochemistry, University of California, Riverside, Riverside, CA 92521, USA.
David A JohnsonDivision of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521, USA.
Han XiaoDivision of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521, USA.
Yinsheng WangDepartment of Chemistry, University of California, Riverside, Riverside, CA 92521, USA.
Shankar SubramaniamDepartments of Bioengineering, Cellular and Molecular Medicine, and Computer Science and Engineering, University of California, San Diego, San Diego, CA 92093, USA.
Shu ChienDepartment of Medicine, University of California, San Diego, San Diego, CA 92093, USA.
John Y-J ShyyDepartment of Medicine, University of California, San Diego, San Diego, CA 92093, USA. jshyy@ucsd.edu.
University of California, Riverside · USUniversity of San Diego · USUniversity of California San Diego · US

Funding

Systems Biology Analyses for Hemodynamic Regulation of Vascular HomeostasisR01HL108735 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHIEN, SHU, SHYY, JOHN YJ · 2012 to 2024
$13.4M
NHLBI NIH HHS R01 HL108735
6 · The paper itself

Abstract

Adenosine monophosphate (AMP)-activated protein kinase (AMPK) acts as a master regulator of cellular energy homeostasis by directly phosphorylating metabolic enzymes and nutrient transporters and by indirectly promoting the transactivation of nuclear genes involved in mitochondrial biogenesis and function. We explored the mechanism of AMPK-mediated induction of gene expression. We identified AMPK consensus phosphorylation sequences in three proteins involved in nucleosome remodeling: DNA methyltransferase 1 (DNMT1), retinoblastoma binding protein 7 (RBBP7), and histone acetyltransferase 1 (HAT1). DNMT1 mediates DNA methylation that limits transcription factor access to promoters and is inhibited by RBBP7. Acetylation of histones by HAT1 creates a more relaxed chromatin-DNA structure that favors transcription. AMPK-mediated phosphorylation resulted in the activation of HAT1 and inhibition of DNMT1. For DNMT1, this inhibition was both a direct effect of phosphorylation and the result of increased interaction with RBBP7. In human umbilical vein cells, pharmacological AMPK activation or pulsatile shear stress triggered nucleosome remodeling and decreased cytosine methylation, leading to increased expression of nuclear genes encoding factors involved in mitochondrial biogenesis and function, such as peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), transcription factor A (Tfam), and uncoupling proteins 2 and 3 (UCP2 and UCP3). Similar effects were seen in the aortas of mice given pharmacological AMPK activators, and these effects required AMPK2α. These results enhance our understanding of AMPK-mediated mitochondrial gene expression through nucleosome remodeling.

Indexed as

Organelle BiogenesisAMP-Activated Protein KinasesCells, CulturedChromatin Assembly and DisassemblyDNA-Binding ProteinsDNA (Cytosine-5-)-Methyltransferase 1DNA MethylationHistone AcetyltransferasesHumansImmunoblottingMitochondrial ProteinsNucleosomesPeroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPhosphorylationPromoter Regions, GeneticProtein BindingAMP-Activated Protein KinasesDNA-Binding ProteinsDNA (Cytosine-5-)-Methyltransferase 1DNMT1 protein, humanHistone Acetyltransferaseshistone acetyltransferase type B complexMitochondrial ProteinsNucleosomesPeroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPPARGC1A protein, humanRBBP7 protein, humanRetinoblastoma-Binding Protein 7TFAM protein, humanTranscription FactorsUCP2 protein, humanUCP3 protein, humanUncoupling Protein 2Uncoupling Protein 3

Identifiers

PMID28143904
PMCPMC5830108
OpenAlexW2584674676

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.