ArticleJournal of lipid research2017
Raman imaging of macrophages incubated with triglyceride-enriched oxLDL visualizes translocation of lipids between endocytic vesicles and lipid droplets.
Article in Journal of lipid research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Multimodal Raman and Fluorescence Lifetime Imaging: A New Analytical Approach for Resolving Timing-Dependent Metabolic Recovery in Endothelial Dysfunction.Analytical chemistry · 2026Article
- Predicting targeted- and immunotherapeutic response outcomes in melanoma with single-cell Raman spectroscopy and AI.bioRxiv : the preprint server for biology · 2026Article
- Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging.Molecules (Basel, Switzerland) · 2026Article
- Selective Tumor Hypoxia Targeting Using M75 Antibody Conjugated Photothermally Active MoOACS omega · 2023Article
- Monitoring the macrophage response towards biomaterial implants using label-free imaging.Materials today. Bio · 2023Article
- Analytical Techniques for Single-Cell Biochemical Assays of Lipids.Annual review of biomedical engineering · 2023Review
- Raman Spectroscopy for Chemical Biology Research.Journal of the American Chemical Society · 2022Review
- Raman microspectroscopy reveals unsaturation heterogeneity at the lipid droplet level and validates anFrontiers in endocrinology · 2022Article
- Lipidome profiling with Raman microspectroscopy identifies macrophage response to surface topographies of implant materials.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Molecular imaging analysis of microvesicular and macrovesicular lipid droplets in non-alcoholic fatty liver disease by Raman microscopy.Scientific reports · 2020Article
- Investigating Origins of FLIm Contrast in Atherosclerotic Lesions Using Combined FLIm-Raman Spectroscopy.Frontiers in cardiovascular medicine · 2020Article
- Tracking intracellular uptake and localisation of alkyne tagged fatty acids using Raman spectroscopy.Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Raman spectroscopic imaging was used to investigate the uptake of oxidized LDLs (oxLDLs) by human macrophages. To better understand the endocytic pathway and the intracellular fate of modified lipoproteins is of foremost interest with regard to the development of atherosclerotic plaques. To obtain information on the storage process of lipids caused by oxLDL uptake, Raman spectroscopic imaging was used because of its unique chemical specificity, especially for lipids. For the present study, a protocol was established to incorporate deuterated tripalmitate into oxLDL. Subsequently, human THP-1 macrophages were incubated for different time points and their chemical composition was analyzed using Raman spectroscopic imaging. β-Carotene was found to be a reliable marker molecule for the uptake of lipoproteins into macrophages. In addition, lipoprotein administration led to small endocytic vesicles with different concentrations of deuterated lipids within the cells. For the first time, the translocation of deuterated lipids from endocytic vesicles into lipid droplets over time is reported in mature human THP-1 macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.