ArticleParasites & vectors2017
Gastropod-derived haemocyte extracellular traps entrap metastrongyloid larval stages of Angiostrongylus vasorum, Aelurostrongylus abstrusus and Troglostrongylus brevior.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed.
- Haemocyte-Derived Innate Immune Reactions of the Giant African Snail (Animals : an open access journal from MDPI · 2026Article
- Proteomic profiles of Lissachatina (Heterobranchia) and Pomacea (Caenogastropoda) snails infected with Angiostrongylus cantonensis using 4D label-free quantitative analysis.PLoS neglected tropical diseases · 2025Article
- Radiolabeling of Angiostrongylus vasorum- and Crenosoma striatum larvae: a novel method using PET/CT to unveil larval migration in the gastropod intermediate host (Lissachatina fulica).Parasites & vectors · 2025Article
- Infection withParasitology · 2025Article
- Organ Tropism ofPathogens (Basel, Switzerland) · 2024Article
- Extracellular DNA traps in a ctenophore demonstrate immune cell behaviors in a non-bilaterian.Nature communications · 2024Article
- A Structural Analysis of Host-Parasite Interactions inPathogens (Basel, Switzerland) · 2023Article
- The Eco-Immunological Relevance of the Anti-Oxidant Response in Invasive Molluscs.Antioxidants (Basel, Switzerland) · 2023Review
- Reaction ofPathogens (Basel, Switzerland) · 2023Article
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- The Expanding Role of Extracellular Traps in Inflammation and Autoimmunity: The New Players in Casting Dark Webs.International journal of molecular sciences · 2022Review
- Tamoxifen triggers theFrontiers in veterinary science · 2022Article
- A Dissenters' View on AppleSnail Immunobiology.Frontiers in immunology · 2022Review
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- Angiostrongylosis in Animals and Humans in Europe.Pathogens (Basel, Switzerland) · 2021Review
- Autochthonous Angiostrongylus cantonensis, Angiostrongylus vasorum and Aelurostrongylus abstrusus infections in native terrestrial gastropods from the Macaronesian Archipelago of Spain.Parasitology research · 2021Article
- CanineBiology · 2021Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundPhagocyte-derived extracellular traps (ETs) were recently demonstrated mainly in vertebrate hosts as an important effector mechanism against invading parasites. In the present study we aimed to characterize gastropod-derived invertebrate extracellular phagocyte trap (InEPT) formation in response to larval stages of important canine and feline metastrongyloid lungworms. Gastropod haemocytes were isolated from the slug species Arion lusitanicus and Limax maximus, and the snail Achatina fulica, and exposed to larval stages of Angiostrongylus vasorum, Aelurostrongylus abstrusus and Troglostrongylus brevior and investigated for gastropod-derived InEPT formation.
resultsPhase contrast as well as scanning electron microscopy (SEM) analyses of lungworm larvae-exposed haemocytes revealed ET-like structures to be extruded by haemocytes thereby contacting and ensnaring the parasites. Co-localization studies of haemocyte-derived extracellular DNA with histones and myeloperoxidase in larvae-entrapping structures confirmed classical characteristics of ETs. In vivo exposure of slugs to A. vasorum larvae resulted in InEPTs being extruded from haemocytes in the slug mucous extrapallial space emphasizing the pivotal role of this effector mechanism against invasive larvae. Functional larval entrapment assays demonstrated that almost half of the haemocyte-exposed larvae were contacted or even immobilized by released InEPTs. Overall, as reported for mammalian-derived ETs, different types of InEPTs were here observed, i.e. aggregated, spread and diffused InEPTs.
conclusionsTo our knowledge, this study represents the first report on metastrongyloid lungworm-triggered ETosis in gastropods thereby providing evidence of early mollusc host innate immune reactions against invading larvae. These findings will contribute to the better understanding on complex parasite-intermediate host interactions since different gastropod species bear different transmitting capacities for metastrongyloid infections.
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