ArticleBMC plant biology2017
Conservation and diversification of the miR166 family in soybean and potential roles of newly identified miR166s.
Article in BMC plant biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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35 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Frontiers in plant science · 2022Pooled it
- Small but big player: the important role of microRNAs in legume crops.Molecular genetics and genomics : MGG · 2026Review
- Rhizospheric soil alter extracellular vesicles in the roots ofFrontiers in genetics · 2026Article
- Genome-Wide Identification of theGenes · 2025Article
- Roles of microRNAs and histone modifications in enhancing stress tolerance in soybean and their applications in molecular breeding.Breeding science · 2025Article
- Detection of closely linked QTLs and candidate genes controlling germination indices in response to drought and salinity stresses in barley.Scientific reports · 2024Article
- Mechanism of action of microRNA166 on nitric oxide in alfalfa (Medicago sativa L.) under drought stress.BMC genomics · 2024Article
- Identification and Expression Analysis of the Nucleotidyl Transferase Protein (NTP) Family in Soybean (International journal of molecular sciences · 2024Article
- Characterization of VvmiR166s-Target Modules and Their Interaction Pathways in Modulation of Gibberellic-Acid-Induced Grape Seedless Berries.International journal of molecular sciences · 2023Article
- Article
- Dynamic Regulation of Grapevine's microRNAs in Response to Mycorrhizal Symbiosis and High Temperature.Plants (Basel, Switzerland) · 2023Article
- Comprehensive, integrative genomic analysis of microRNA expression profiles in different tissues of two wheat cultivars with different traits.Functional & integrative genomics · 2022Article
- Mining the Roles of Cucumber DUF966 Genes in Fruit Development and Stress Response.Plants (Basel, Switzerland) · 2022Article
- Identification and profiling of conserved microRNAs in different developmental stages of crown imperial (Fritillaria imperialis L.) using high-throughput sequencing.Molecular biology reports · 2022Article
- Conservation and Diversity of miR166 Family Members From Highbush Blueberry (Frontiers in genetics · 2022Article
- Genome-wide identification and comprehensive analyses ofFrontiers in plant science · 2022Article
- microRNA 166: an evolutionarily conserved stress biomarker in land plants targeting HD-ZIP family.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Characterization of microRNAs from neem (3 Biotech · 2021Article
- Sequence and functional analysis of MIR319 promoter homologs from Brassica juncea reveals regulatory diversification and altered expression under stress.Molecular genetics and genomics : MGG · 2021Article
- Edible plant-derived exosomal microRNAs: Exploiting a cross-kingdom regulatory mechanism for targeting SARS-CoV-2.Toxicology and applied pharmacology · 2021Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundmicroRNA166 (miR166) is a highly conserved family of miRNAs implicated in a wide range of cellular and physiological processes in plants. miR166 family generally comprises multiple miR166 members in plants, which might exhibit functional redundancy and specificity. The soybean miR166 family consists of 21 members according to the miRBase database. However, the evolutionary conservation and functional diversification of miR166 family members in soybean remain poorly understood.
resultsWe identified five novel miR166s in soybean by data mining approach, thus enlarging the size of miR166 family from 21 to 26 members. Phylogenetic analyses of the 26 miR166s and their precursors indicated that soybean miR166 family exhibited both evolutionary conservation and diversification, and ten pairs of miR166 precursors with high sequence identity were individually grouped into a discrete clade in the phylogenetic tree. The analysis of genomic organization and evolution of MIR166 gene family revealed that eight segmental duplications and four tandem duplications might occur during evolution of the miR166 family in soybean. The cis-elements in promoters of MIR166 family genes and their putative targets pointed to their possible contributions to the functional conservation and diversification. The targets of soybean miR166s were predicted, and the cleavage of ATHB14-LIKE transcript was experimentally validated by RACE PCR. Further, the expression patterns of the five newly identified MIR166s and 12 target genes were examined during seed development and in response to abiotic stresses, which provided important clues for dissecting their functions and isoform specificity.
conclusionThis study enlarged the size of soybean miR166 family from 21 to 26 members, and the 26 soybean miR166s exhibited evolutionary conservation and diversification. These findings have laid a foundation for elucidating functional conservation and diversification of miR166 family members, especially during seed development or under abiotic stresses.
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