Evidence map›Paper›PMID 28140749›Full record

ArticleBioengineered2017

Aberrant methylated EDNRB can act as a potential diagnostic biomarker in sporadic colorectal cancer while KISS1 is controversial.

Reza Mousavi Ardehaie, Shahriar Hashemzadeh, Shahin Behrouz Sharif, Morteza Ghojazadeh, Ladan Teimoori-Toolabi, Ebrahim Sakhinia

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineered, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 18 citations in OpenAlex.

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  7. Methylation as a critical epigenetic process during tumor progressions among Iranian population: an overview.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Reza Mousavi Ardehaiea Department of Biochemistry and Clinical Laboratory , Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , Iran.
Shahriar Hashemzadehb Department of General & Vascular Surgery , Tabriz University of Medical Sciences , Tabriz , Iran.
Shahin Behrouz Sharifa Department of Biochemistry and Clinical Laboratory , Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , Iran.
Morteza Ghojazadehd Liver and Gastrointestinal Disease Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
Ladan Teimoori-Toolabie Molecular Medicine Department , Pasteur Institute of Iran , Tehran , Iran.
Ebrahim Sakhiniaa Department of Biochemistry and Clinical Laboratory , Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , Iran.
Tabriz University of Medical Sciences · IRPasteur Institute of Iran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cancers are among the most serious threats of human health worldwide. Survival and mortality rates of colorectal cancer (CRC) strongly depend on the early diagnosis. The aberrant methylation pattern of genes as a diagnostic biomarker can serve as a practical option for timely detection and contribute subsequently to the enhancement of survival rate in CRC patients, since methylation changes are not only frequent but also can occur in initial tumorogenesis stages. It has been indicated that EDNRB and KISS1 genes are hypermethylated through progression and development of CRC. In current study, after extraction of genomic DNA from 45 paired tumor and adjacent non-cancerous tissue samples and treatment with bisulfite conversion, the methylation status of EDNRB and KISS1 CpG rich regions were assessed quantitatively using MS-HRM assay to determine practicability of these aberrant methylations for diagnosis of sporadic CRC and its discrimination from corresponding normal tissues. The results showed that the methylation distribution differences, comparing tumor tissues with their adjacent non-cancerous tissues, were statistically significant in all selected locations within EDNRB gene promoter (P < 0.001); they had also some correlations with tumor stage and grade. Nonetheless, methylation distribution in KISS1 gene CpG rich region revealed no statistically significant differences between CRC and adjacent non-cancerous tissues (P = 0.060). Overall, it can be concluded that aberrant methylated EDNRB can be a promising potential diagnostic biomarker for CRC, while KISS1 is controversial and needs to be more investigated.

Indexed as

Biomarkers, TumorColorectal NeoplasmsDNA MethylationGenetic Predisposition to DiseaseHumansIranKisspeptinsMutationPolymorphism, Single NucleotidePrevalenceReceptor, Endothelin BReproducibility of ResultsRisk FactorsSensitivity and SpecificityBiomarkers, TumorEDNRB protein, humanKISS1 protein, humanKisspeptinsReceptor, Endothelin Bbiomarkercolorectal cancerEDNRBhypermethylationKISS1MS-HRM assay

Identifiers

PMID28140749
PMCPMC5639868
OpenAlexW2584864985

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.