ArticleScientific reports2017
Thicker three-dimensional tissue from a "symbiotic recycling system" combining mammalian cells and algae.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 69 citations in OpenAlex.
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- Strength in diversity: unlocking the full potential of engineered living materials with multistrain collaboration.FEMS microbiology reviews · 2025Review
- Microalgae and Human Disorders: Mechanistic Insights and Therapeutic Potential.Current topics in medicinal chemistry · 2025Review
- Recombinant lactate-assimilating cyanobacteria reduce high-concentration culture-associated cytotoxicity in mammalian cells.Archives of microbiology · 2024Article
- 3D Bioprinting in Microgravity: Opportunities, Challenges, and Possible Applications in Space.Advanced healthcare materials · 2023Article
- Microalgae share key features with human erythrocytes and can safely circulate through the vascular system in mice.Applied microbiology and biotechnology · 2023Article
- Repurposing biomedical muscle tissue engineering for cellular agriculture: challenges and opportunities.Trends in biotechnology · 2023Review
- Circular cell culture for sustainable food production using recombinant lactate-assimilating cyanobacteria that supplies pyruvate and amino acids.Archives of microbiology · 2023Article
- Microfluidic encapsulation of photosynthetic cyanobacteria in hydrogel microparticles augments oxygen delivery to rescue ischemic myocardium.Journal of bioscience and bioengineering · 2023Article
- A circular cell culture system using microalgae and mammalian myoblasts for the production of sustainable cultured meat.Archives of microbiology · 2022Article
- Bioengineering Outlook on Cultivated Meat Production.Micromachines · 2022Review
- Perspectives on scaling production of adipose tissue for food applications.Biomaterials · 2022Article
- The Effect of Gracilaria Corticata and Scenedesmus Acuminates Extract Mixture on the Healing of Wounds Contaminated with Staphylococcus in the Rat Model.Archives of academic emergency medicine · 2022Article
- Microalgal culture in animal cell waste medium for sustainable 'cultured food' production.Archives of microbiology · 2021Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, we report an in vitro co-culture system that combines mammalian cells and algae, Chlorococcum littorale, to create a three-dimensional (3-D) tissue. While the C2C12 mouse myoblasts and rat cardiac cells consumed oxygen actively, intense oxygen production was accounted for by the algae even in the co-culture system. Although cell metabolism within thicker cardiac cell-layered tissues showed anaerobic respiration, the introduction of innovative co-cultivation partially changed the metabolism to aerobic respiration. Moreover, the amount of glucose consumption and lactate production in the cardiac tissues and the amount of ammonia in the culture media decreased significantly when co-cultivated with algae. In the cardiac tissues devoid of algae, delamination was observed histologically, and the release of creatine kinase (CK) from the tissues showed severe cardiac cell damage. On the other hand, the layered cell tissues with algae were observed to be in a good histological condition, with less than one-fifth decline in CK release. The co-cultivation with algae improved the culture condition of the thicker tissues, resulting in the formation of 160 μm-thick cardiac tissues. Thus, the present study proposes the possibility of creating an in vitro "symbiotic recycling system" composed of mammalian cells and algae.
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