ArticleSaudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association
Diagnostic roles of calretinin in hirschsprung disease: A comparison to neuron-specific enolase.
Article in Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 24 citations in OpenAlex.
- Full-Thickness Rectal Biopsy in Children Suspected of Having Hirschsprung's Disease: The Inconclusive Biopsy.Children (Basel, Switzerland) · 2023Article
- Rectal Biopsy for Hirschsprung's Disease: A Multicentre Study Involving Biopsy Technique, Pathology and Complications.Children (Basel, Switzerland) · 2023Article
- Rare presentation and retrograde diagnosis of total colonic aganglionosis in a female infant: a case report.Journal of medical case reports · 2023Article
- Calretinin immunohistochemical staining in Hirschsprung's disease: An institutional experience.Northern clinics of Istanbul · 2021Article
- Pathogenesis of chronic constipation in a Polish group of paediatric patients - an attempt to create the optimal histopathological diagnostic protocol.Przeglad gastroenterologiczny · 2019Article
- Histopathologic and immunohistochemical findings in congenital anorectal malformations.Medicine · 2018Article
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4 authors at 3 institutions in 1 country.
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Abstract
BACKGROUND/
aimDiagnosis of Hirschsprung's disease (HD) can be hard and requires good experience, principally for pathologists who infrequently encounter the disease. However, diagnosis is not always possible with hematoxylin and eosin (H and E) because staining has limitations in the identification of immature ganglion cells in neonates and the submucosal area.
aimTo assess the diagnostic role of calretinin immunostaining in HD in comparison to neuron-specific enolase. PATIENTS AND
methodsFormalin-fixed paraffin tissue blocks of full-thickness distal colonic and rectal biopsies for 48 patients who clinically presented with symptoms suspicious for HD were collected for the period from December 2012 to January 2016. All biopsies were already studied by routine H and E histopathological examination for the presence or absence of ganglion cells. Further confirmation of ganglion cells and nerve fibers was performed by immunohistochemical study for neuron-specific enolase and calretinin, respectively, in a private pathology laboratory.
resultsAccording to the histopathological assessment, cases with absent ganglionic cells were considered to be HD, which comprised 40 cases out of the total 48 cases. The mean age for HD cases was 19.43 months. The male-to-female ratio in HD cases was 2.34:1. All HD cases showed negative expression of calretinin in small nerve fibers of the lamina propria, musularis mucosae, and submucosa, and negative expression of neuron-specific enolase in ganglionic cells. The sensitivity, specificity, positive predictive value, and negative predictive values for both the markers in the confirmation of diagnosis of HD were all 100%.
conclusionCalretinin immunostaining, similar to that of neuron-specific enolase, is a highly sensitive and specific diagnostic aid to histopathological examination in suspected HD.
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