ArticleAmerican journal of reproductive immunology (New York, N.Y. : 1989)2017
Time course analysis of RNA quality in placenta preserved by RNAlater or flash freezing.
Article in American journal of reproductive immunology (New York, N.Y. : 1989), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Current approaches and developments in transcript profiling of the human placenta.Human reproduction update · 2020Pooled it
- Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes.BMC biotechnology · 2025Article
- Optimizing Tissue Sampling Timing for Accurate Gene Expression Analysis.International journal of molecular sciences · 2025Article
- Comparison Between Two Methodologies of Sample Preservation for RNA Extraction in Naturally Delivered Ovine Placenta.Animals : an open access journal from MDPI · 2025Article
- DNA/RNA Preservation in Glacial Snow and Ice Samples.Frontiers in microbiology · 2022Article
- A new pipeline for clinico-pathological and molecular placental research utilizing FFPE tissues.Placenta · 2021Article
- Comparative Transcriptomic Analysis of Staphylococcus aureus Associated with Periprosthetic Joint Infection under in Vivo and in Vitro Conditions.The Journal of molecular diagnostics : JMD · 2021Article
- Transcriptomic analysis of equine chorioallantois reveals immune networks and molecular mechanisms involved in nocardioform placentitis.Veterinary research · 2021Article
- Human PapillomavirusPathogens (Basel, Switzerland) · 2020Article
- Mechanisms and Measurement of Changes in Gene Expression.Biological research for nursing · 2018Article
- Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution.PloS one · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
problemPreservation of biospecimen quality is critical to accurately and reliably assessing genes and proteins. We evaluated the effect of preparation method and storage duration on RNA quality in placenta and decidua. METHOD OF STUDY: Aliquots of nine placentas and decidua were placed in RNAlater
resultsAt both time points, RL placenta had RIN and housekeeping gene Ct values similar to baseline. However, FF placenta had significantly lower RIN and higher Ct values at 1 and 8-10 months. In RL and FF decidua, RIN was unchanged from baseline.
conclusionWe found RNAlater more effectively and consistently preserved placenta, compared to flash freezing. However, for decidua, which is less dense than placenta, both modes yielded comparable RNA integrity over time.
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Registered trials
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