ArticleScience advances2016
Adhesion mechanisms of curli subunit CsgA to abiotic surfaces.
Article in Science advances, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Charged agar surfaces affectBiofilm · 2026Article
- Adhesion Mechanisms of Amyloid-Like Oligomer Monolayers Enabling Stable Surface Modification for Inert Polymers.JACS Au · 2026Article
- Roles of micro/nanoplastics in the spread of antimicrobial resistance through conjugative gene transfer.Nature communications · 2025Article
- Review
- Comprehensive review of bacterial death mechanism on nanopillared nanostructured surfaces.Biophysical reviews · 2025Review
- Effect of shear rate on earlyBiofilm · 2024Article
- Natural Antimicrobial Mixtures Disrupt Attachment and Survival ofFoods (Basel, Switzerland) · 2023Article
- Realization of Amyloid-like Aggregation as a Common Cause for Pathogenesis in Diseases.Life (Basel, Switzerland) · 2023Review
- Influence of Surface Roughness, Nanostructure, and Wetting on Bacterial Adhesion.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Recent advances in biomimetic hemostatic materials.Materials today. Bio · 2023Review
- Sticking to the Subject: Multifunctionality in Microbial Adhesins.Journal of fungi (Basel, Switzerland) · 2023Review
- Protein-Based Biological Materials: Molecular Design and Artificial Production.Chemical reviews · 2023Review
- Roles of Tryptophan and Charged Residues on the Polymorphisms of Amyloids Formed by K-Peptides of Hen Egg White Lysozyme Investigated through Molecular Dynamics Simulations.International journal of molecular sciences · 2023Article
- Dynamic single cell analysis in a proximal-tubule-on-chip reveals heterogeneous epithelial colonization strategies of uropathogenicFEMS microbes · 2023Article
- Anti-biofilm activity of caffeine against uropathogenic E. coli is mediated by curli biogenesis.Scientific reports · 2022Article
- Molecular-level insights into the surface-induced assembly of functional bacterial amyloid.Biophysical journal · 2022Article
- The CRISPR-Cas System Differentially Regulates Surface-Attached and Pellicle Biofilm in Salmonella enterica Serovar Typhimurium.Microbiology spectrum · 2022Article
- Functional Bacterial Amyloids: Understanding Fibrillation, Regulating Biofilm Fibril Formation and Organizing Surface Assemblies.Molecules (Basel, Switzerland) · 2022Review
- Amyloid-Like Protein Aggregation Toward Pesticide Reduction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Manipulating Bacterial Biofilms Using Materiobiology and Synthetic Biology Approaches.Frontiers in microbiology · 2022Review
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Authors and funding
3 authors.
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Abstract
Curli fibers are functional amyloids that play a key role in biofilm structure and adhesion to various surfaces. Strong bioinspired adhesives comprising curli fibers have recently been created; however, the mechanisms curli uses to attach onto abiotic surfaces are still uncharacterized. Toward a materials-by-design approach for curli-based adhesives and multifunctional materials, we examine curli subunit adsorption onto graphene and silica surfaces through atomistic simulation. We find that both structural features and sequence influence adhesive strength, enabling the CsgA subunit to adhere strongly to both polar and nonpolar surfaces. Specifically, flexible regions facilitate adhesion to both surfaces, charged and polar residues (Arg, Lys, and Gln) enable strong interactions with silica, and six-carbon aromatic rings (Tyr and Phe) adsorb strongly to graphene. We find that adsorption not only lowers molecular mobility but also leads to loss of secondary structure, factors that must be well balanced for effective surface attachment. Both events appear to propagate through the CsgA structure as correlated motion between clusters of residues, often H-bonded between rows on adjacent β strands. To quantify this, we present a correlation analysis approach to detecting collective motion between residue groups. We find that certain clusters of residues have a higher impact on the stability of the rest of the protein structure, often polar and bulky groups within the helix core. These findings lend insight into bacterial adhesion mechanisms and reveal strategies for theory-driven design of engineered curli fibers that harness point mutations and conjugates for stronger adhesion.
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