Evidence map›Paper›PMID 28137878›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2017

Dynamics of GreB-RNA polymerase interaction allow a proofreading accessory protein to patrol for transcription complexes needing rescue.

Larry E Tetone, Larry J Friedman, Melisa L Osborne, Harini Ravi, Scotty Kyzer, Sarah K Stumper, Rachel A Mooney, Robert Landick, Jeff Gelles

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

  1. RfaH licenses RNA polymerase for long-range transcription.bioRxiv : the preprint server for biology · 2026
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  5. Review
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  7. Review
  8. RNA polymerase sliding on DNA can couple the transcription of nearby bacterial operons.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  9. Article
  10. Article
  11. Article
  12. Article
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  16. Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Larry E TetoneDepartment of Biochemistry, Brandeis University, Waltham, MA 02454.
Larry J FriedmanDepartment of Biochemistry, Brandeis University, Waltham, MA 02454.
Melisa L OsborneDepartment of Biochemistry, Brandeis University, Waltham, MA 02454.
Harini RaviDepartment of Biochemistry, Brandeis University, Waltham, MA 02454.
Scotty KyzerDepartment of Biochemistry, University of Wisconsin, Madison, WI 53706.
Sarah K StumperDepartment of Biochemistry, Brandeis University, Waltham, MA 02454.
Rachel A MooneyDepartment of Biochemistry, University of Wisconsin, Madison, WI 53706.
Robert LandickDepartment of Biochemistry, University of Wisconsin, Madison, WI 53706.
Jeff GellesDepartment of Biochemistry, Brandeis University, Waltham, MA 02454; gelles@brandeis.edu.ORCID 0000-0001-7910-3421

Funding

STRUCTURE/FUNCTION OF TRANSCRIPTION COMPLEX RNA HAIRPINSR01GM038660 · NIGMS · WASHINGTON UNIVERSITY · PI Robert Landick · 1992 to 2026
$12.2M
Single-molecule visualization of transcription regulation mechanismsR01GM081648 · NIGMS · BRANDEIS UNIVERSITY · PI JEFF GELLES · 2007 to 2026
$7.4M
Macromolecular Structure MechanismT32GM007596 · NIGMS · BRANDEIS UNIVERSITY · PI OPRIAN, DANIEL D · 1985 to 2019
$4.5M
Quantitative Biology: a Graduate Curriculum Linking the Physical and Biomedical ST32EB009419 · NIBIB · BRANDEIS UNIVERSITY · PI GELLES, JEFF, KONDEV, JANE · 2009 to 2018
$2.8M
BIOPHYSICS AND ENZYMOLOGY OF KINESIN MOVEMENTR01GM043369 · NIGMS · BRANDEIS UNIVERSITY · PI GELLES, JEFF · 1991 to 2001
$587k
NIBIB NIH HHS T32 EB009419NIGMS NIH HHS R01 GM038660NIGMS NIH HHS R01 GM043369NIGMS NIH HHS R01 GM081648NIGMS NIH HHS T32 GM007596
6 · The paper itself

Abstract

The secondary channel (SC) of multisubunit RNA polymerases (RNAPs) allows access to the active site and is a nexus for the regulation of transcription. Multiple regulatory proteins bind in the SC and reprogram the catalytic activity of RNAP, but the dynamics of these factors' interactions with RNAP and how they function without cross-interference are unclear. In

Indexed as

Gene Expression Regulation, BacterialTranscription, GeneticBenzenesulfonatesBinding SitesCarbocyaninesComputer SimulationDNA, BacterialDNA-Directed RNA PolymerasesEscherichia coliEscherichia coli ProteinsFluorescent DyesModels, GeneticModels, MolecularMonte Carlo MethodProtein BindingSingle Molecule ImagingBenzenesulfonatesCarbocyaninesCy3B N-hydroxysuccinimide esterDNA, BacterialDNA-Directed RNA PolymerasesEscherichia coli ProteinsFluorescent DyesGreB protein, E colirpoB protein, E coliTranscriptional Elongation Factorsbacktrackingsecondary channeltotal internal reflection fluorescencetranscription elongation

Identifiers

PMID28137878
PMCPMC5320998

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.