Evidence map›Paper›PMID 28137312›Full record

ArticleBMC molecular biology2017

Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5.

Ling Zhang, Yunhao Liu, Wei Li, Qiaoling Zhang, Yanwei Li, Junpin Liu, Jie Min, Chaofan Shuang, Shizheng Song, Zhibing Zhang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
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  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Ling ZhangSchool of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, Hubei, China. zhangling@wust.edu.cn.
Yunhao LiuSchool of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, Hubei, China.
Wei LiDepartment of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, 23298, USA.
Qiaoling ZhangCentral China Normal University, Wuhan, Hubei, 430000, China.
Yanwei LiDepartment of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, 23298, USA.
Junpin LiuDepartment of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, 23298, USA.
Jie MinSchool of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, Hubei, China.
Chaofan ShuangSchool of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, Hubei, China.
Shizheng SongSchool of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, Hubei, China.
Zhibing ZhangDepartment of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, 23298, USA. zhibing.zhang@vcuhealth.org.
Wuhan University of Science and Technology · CNVirginia Commonwealth University · USCentral China Normal University · CNWellesley College · USWuhan Prevention and Treatment Center for Occupational Diseases · CN

Funding

Dissection of the Structural Basis of MEIG1 in Assembling Sperm FlagellaR01HD076257 · NICHD · WAYNE STATE UNIVERSITY · PI ZHANG, ZHIBING · 2013 to 2017
$1.5M
The Role of Transcription Factor S-SOX5 in Male Fertility and Sperm Flagella FormationR03HD090306 · NICHD · WAYNE STATE UNIVERSITY · PI ZHANG, ZHIBING · 2017 to 2018
$145k
NICHD NIH HHS R01 HD076257NICHD NIH HHS R03 HD090306
6 · The paper itself

Abstract

backgroundThe mammalian sperm-associated antigen 16 gene (Spag16) uses alternative promoters to produce two major transcript isoforms (Spag16L and Spag16S) and encode proteins that are involved in the cilia/flagella formation and motility. In silico analysis of both mouse and human SPAG16L promoters reveals the existence of multiple putative SOX5 binding sites. Given that the SOX5 gene encodes a 48-kDa transcription factor (S-SOX5) and the presence of putative SOX5 binding sites at the SPAG16L promoter, regulation of SPAG16L expression by S-SOX5 was studied in the present work.

resultsS-SOX5 activated human SPAG16L promoter activity in the human bronchial epithelia cell line BEAS-2B cells. Mutation of S-SOX5 binding sites abolished the stimulatory effect. Overexpression of S-SOX5 resulted in a significant increase in the abundance of SPAG16L transcripts whereas silencing of S-SOX5 by RNAi largely reduced the SPAG16L expression. Chromatin immunoprecipitation assays showed that S-SOX5 directly interacts with the SPAG16L promoter.

conclusionS-SOX5 regulates transcription of human SPAG16L gene via directly binding to the promoter of SPAG16L. It has been reported that expression of sperm-associated antigen 6 (SPAG6), encoding another axonemal protein, is activated by S-SOX5. Therefore, S-SOX5 may regulate formation of motile cilia/flagella through globally mediating expression of genes encoding axonemal proteins.

Indexed as

Transcriptional ActivationCell LineHumansMicrotubule-Associated ProteinsMutationPromoter Regions, GeneticProtein IsoformsRespiratory MucosaRNA InterferenceRNA, MessengerSOXD Transcription FactorsMicrotubule-Associated ProteinsProtein IsoformsRNA, MessengerSOX5 protein, humanSOXD Transcription FactorsSPAG16 protein, humanCentral apparatusCiliaSPAG16LS-SOX5Transcriptional regulation

Identifiers

PMID28137312
PMCPMC5282894
OpenAlexW2580550886

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.