Evidence map›Paper›PMID 28135906›Full record

ArticleCell cycle (Georgetown, Tex.)2017

GAK is phosphorylated by c-Src and translocated from the centrosome to chromatin at the end of telophase.

Kohshiro Fukushima, Mian Wang, Yoko Naito, Toshihiro Uchihashi, Yorika Kato, Satomi Mukai, Norikazu Yabuta, Hiroshi Nojima

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell cycle (Georgetown, Tex.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Kohshiro Fukushimaa Department of Molecular Genetics , Research Institute for Microbial Diseases, Osaka University , Yamadaoka, Suita, Osaka , Japan.
Mian Wanga Department of Molecular Genetics , Research Institute for Microbial Diseases, Osaka University , Yamadaoka, Suita, Osaka , Japan.
Yoko Naitoa Department of Molecular Genetics , Research Institute for Microbial Diseases, Osaka University , Yamadaoka, Suita, Osaka , Japan.
Toshihiro Uchihashib First Department of Oral and Maxillofacial Surgery , Graduate School of Dentistry, Osaka University , Yamadaoka, Suita, Osaka , Japan.
Yorika Katoa Department of Molecular Genetics , Research Institute for Microbial Diseases, Osaka University , Yamadaoka, Suita, Osaka , Japan.
Satomi Mukaia Department of Molecular Genetics , Research Institute for Microbial Diseases, Osaka University , Yamadaoka, Suita, Osaka , Japan.
Norikazu Yabutaa Department of Molecular Genetics , Research Institute for Microbial Diseases, Osaka University , Yamadaoka, Suita, Osaka , Japan.
Hiroshi Nojimaa Department of Molecular Genetics , Research Institute for Microbial Diseases, Osaka University , Yamadaoka, Suita, Osaka , Japan.
Osaka University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cyclin G-associated kinase (GAK) harbors a consensus phosphorylation motif (Y412) for c-Src; however, its physiological significance remains elusive. Here, we show that GAK is phosphorylated by c-Src not only at Y412 but also at Y1149. An anti-GAK-pY412 antibody recognized the shifted band of GAK during M phase. Immunofluorescence (IF) showed that GAK-pY412/pY1149 signals were present in the nucleus during interphase, translocated to chromosomes at prophase and prometaphase, moved to centrosomes at metaphase, and finally translocated to chromosomes at the end of telophase, when nuclear membrane formation was almost complete. These subcellular movements of GAK resemble those of DNA licensing factors. Indeed, mass spectrometry identified mini-chromosome maintenance (MCM) 3, an essential component of the DNA licensing system, as one of the association partners of GAK; immunoprecipitation-mediated Western blotting confirmed their association in vivo. These results suggest that the c-Src_GAK_MCM axis plays an important role in cell cycle progression through control of the DNA replication licensing system.

Indexed as

TelophaseAntibodiesCell NucleusCell ProliferationCentrosomeChromatinHeLa CellsHumansInterphaseIntracellular Signaling Peptides and ProteinsMinichromosome Maintenance Complex Component 2Minichromosome Maintenance Complex Component 3MitosisPhosphorylationProtein BindingProtein Serine-Threonine KinasesAntibodiesChromatinGAK protein, humanIntracellular Signaling Peptides and ProteinsMCM2 protein, humanMCM3 protein, humanMinichromosome Maintenance Complex Component 2Minichromosome Maintenance Complex Component 3Protein Serine-Threonine Kinasessrc-Family Kinasesc-SrcDNA replication licensingGAKMCM3phosphorylation

Identifiers

PMID28135906
PMCPMC5351929
OpenAlexW2583207512

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.