ArticlePloS one2017
Transcriptome Analysis and Differential Gene Expression on the Testis of Orange Mud Crab, Scylla olivacea, during Sexual Maturation.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 54 citations in OpenAlex.
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- Transcriptome Profile and Gene Expression During Different Ovarian Maturation Stages ofTropical life sciences research · 2024Article
- Cell adhesion function was altered during the seasonal regression of the seminiferous epithelium in the mink species Neovison vison.Journal of animal science · 2023Article
- The transcriptional landscape of the giant freshwater prawn: Embryonic development and early sexual differentiation mechanisms.Frontiers in endocrinology · 2022Article
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- Comparative transcriptome profiling of heat stress response of the mangrove crab Scylla serrata across sites of varying climate profiles.BMC genomics · 2021Article
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- The Single-molecule long-read sequencing of Scylla paramamosain.Scientific reports · 2019Article
- Integrative Analysis of Transcriptome and GWAS Data to Identify the Hub Genes Associated With Milk Yield Trait in Buffalo.Frontiers in genetics · 2019Article
- Molecular characterization of putative neuropeptide, amine, diffusible gas and small molecule transmitter biosynthetic enzymes in the eyestalk ganglia of the American lobster, Homarus americanus.Invertebrate neuroscience : IN · 2018Article
- Transcriptome sequencing and molecular markers discovery in the gonads of Portunus sanguinolentus.Scientific data · 2018Article
- Comparative Transcriptome Analysis Provides Insights into Differentially Expressed Genes and Long Non-Coding RNAs between Ovary and Testis of the Mud Crab (Scylla paramamosain).Marine biotechnology (New York, N.Y.) · 2018Article
- Transcriptomic Analysis Reveals Insights on Male Infertility inFrontiers in physiology · 2018Article
- Transcriptome profiling of claw muscle of the mud crab (Scylla paramamosain) at different fattening stages.PloS one · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adequate genetic information is essential for sustainable crustacean fisheries and aquaculture management. The commercially important orange mud crab, Scylla olivacea, is prevalent in Southeast Asia region and is highly sought after. Although it is a suitable aquaculture candidate, full domestication of this species is hampered by the lack of knowledge about the sexual maturation process and the molecular mechanisms behind it, especially in males. To date, data on its whole genome is yet to be reported for S. olivacea. The available transcriptome data published previously on this species focus primarily on females and the role of central nervous system in reproductive development. De novo transcriptome sequencing for the testes of S. olivacea from immature, maturing and mature stages were performed. A total of approximately 144 million high-quality reads were generated and de novo assembled into 160,569 transcripts with a total length of 142.2 Mb. Approximately 15-23% of the total assembled transcripts were annotated when compared to public protein sequence databases (i.e. UniProt database, Interpro database, Pfam database and Drosophila melanogaster protein database), and GO-categorised with GO Ontology terms. A total of 156,181 high-quality Single-Nucleotide Polymorphisms (SNPs) were mined from the transcriptome data of present study. Transcriptome comparison among the testes of different maturation stages revealed one gene (beta crystallin like gene) with the most significant differential expression-up-regulated in immature stage and down-regulated in maturing and mature stages. This was further validated by qRT-PCR. In conclusion, a comprehensive transcriptome of the testis of orange mud crabs from different maturation stages were obtained. This report provides an invaluable resource for enhancing our understanding of this species' genome structure and biology, as expressed and controlled by their gonads.
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