ArticleNature methods2017
One-step generation of conditional and reversible gene knockouts.
Article in Nature methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 88 citations in OpenAlex.
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- Universal conditional knockout approach to multiple species, from fish to human induced pluripotent stem cells.Nucleic acids research · 2026Article
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- The application of organoids in investigating immune evasion in the microenvironment of gastric cancer and screening novel drug candidates.Molecular cancer · 2025Review
- Individualized Pooled CRISPR/Cas9 Screenings Identify CDK2 as a Druggable Vulnerability in a Canine Mammary Carcinoma Patient.Veterinary sciences · 2025Article
- Integrin-activatingProceedings of the National Academy of Sciences of the United States of America · 2025Article
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- An in vitro neurogenetics platform for precision disease modeling in the mouse.Science advances · 2024Article
- An oocyte-specific Cas9-expressing mouse for germline CRISPR/Cas9-mediated genome editing.Genesis (New York, N.Y. : 2000) · 2024Article
- Epidermal growth factor receptor (EGFR) is a target of the tumor-suppressor E3 ligase FBXW7.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- METTL14 is a chromatin regulator independent of its RNA N6-methyladenosine methyltransferase activity.Protein & cell · 2023Article
- One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids.Nature communications · 2023Article
- Review
- Review
- SCON-a Short Conditional intrON for conditional knockout with one-step zygote injection.Experimental & molecular medicine · 2022Article
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- A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression.Development (Cambridge, England) · 2022Article
- Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus.Neural regeneration research · 2022Article
- Persistent DNA damage associated with ATM kinase deficiency promotes microglial dysfunction.Nucleic acids research · 2022Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
Abstract
Loss-of-function studies are key for investigating gene function, and CRISPR technology has made genome editing widely accessible in model organisms and cells. However, conditional gene inactivation in diploid cells is still difficult to achieve. Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid and scalable method for biallelic conditional gene knockouts in diploid or aneuploid cells, such as pluripotent stem cells, 3D organoids and cell lines, by co-delivery of CRISPR-Cas9 and a universal conditional intronic cassette.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.