Evidence map›Paper›PMID 28134300›Full record

ArticleScientific reports2017

Clone and functional analysis of Seryl-tRNA synthetase and Tyrosyl-tRNA synthetase from silkworm, Bombyx mori.

Jingsheng Hu, Jianghai Tian, Fanchi Li, Bin Xue, Jiahuan Hu, Xiaoyu Cheng, Jinxin Li, Weide Shen, Bing Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Jingsheng HuSchool of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
Jianghai TianSchool of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
Fanchi LiSchool of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
Bin XueSchool of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
Jiahuan HuSchool of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
Xiaoyu ChengSchool of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
Jinxin LiSchool of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
Weide ShenSchool of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
Bing LiSchool of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
Soochow University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aminoacyl-tRNA synthetases are the key enzymes for protein synthesis. Glycine, alanine, serine and tyrosine are the major amino acids composing fibroin of silkworm. Among them, the genes of alanyl-tRNA synthetase (AlaRS) and glycyl-tRNA synthetase (GlyRS) have been cloned. In this study, the seryl-tRNA synthetase (SerRS) and tyrosyl-tRNA synthetase (TyrRS) genes from silkworm were cloned. Their full length are 1709 bp and 1868 bp and contain open reading frame (ORF) of 1485 bp and 1575 bp, respectively. RT-PCR examination showed that the transcription levels of SerRS, TyrRS, AlaRS and GlyRS are significantly higher in silk gland than in other tissues. In addition, their transcription levels are much higher in middle and posterior silk gland than in anterior silk gland. Moreover, treatment of silkworms with phoxim, an inhibitor of silk protein synthesis, but not TiO

Indexed as

Amino AcidsAmino Acid SequenceAnimalsBombyxCloning, MolecularDNA, ComplementaryEvolution, MolecularGene ExpressionMetal NanoparticlesOpen Reading FramesPhylogenySerine-tRNA LigaseTitaniumTyrosine-tRNA LigaseAmino AcidsDNA, ComplementarySerine-tRNA LigaseTitaniumtitanium dioxideTyrosine-tRNA Ligase

Identifiers

PMID28134300
PMCPMC5278501
OpenAlexW2582701302

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.