ArticleScientific reports2017
Spheroid arrays for high-throughput single-cell analysis of spatial patterns and biomarker expression in 3D.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Human Stem Cell-Derived Neurospheroids and a Multi-Assay Strategy Provide Brain Cytotoxicity New Insights from ADB-FUBIATA Exposure.Neurotoxicity research · 2026Article
- Evaluation of a human 3D multi-spheroid model derived from SH-SY5Y cells for cytotoxicity testing.Scientific reports · 2026Article
- The Size of Glioblastoma Spheroids Influences Patterns of Invasion and Temozolomide Efficacy.Tissue engineering. Part C, Methods · 2026Article
- Size-dependent invasion and therapeutic phenotype of 42MGBA glioblastoma spheroids.bioRxiv : the preprint server for biology · 2025Article
- PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies.Scientific reports · 2025Article
- The role of mesenchymal cells in cholangiocarcinoma.Disease models & mechanisms · 2024Article
- Cost-effective optimized method to process 3D tumoral spheroids in microwell arrays for immunohistochemistry analysis.Journal of medicine and life · 2024Article
- The Transformative Role of 3D Culture Models in Triple-Negative Breast Cancer Research.Cancers · 2024Review
- 3D human stem-cell-derived neuronal spheroids forCurrent research in toxicology · 2024Article
- Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics.Scientific reports · 2023Article
- Cerebral Organoid Arrays for Batch Phenotypic Analysis in Sections and Three Dimensions.International journal of molecular sciences · 2023Article
- OrBITS: label-free and time-lapse monitoring of patient derived organoids for advanced drug screening.Cellular oncology (Dordrecht, Netherlands) · 2023Article
- Changes of protein expression during tumorosphere formation of small cell lung cancer circulating tumor cells.Oncology research · 2023Article
- Evaluation of X-ray and carbon-ion beam irradiation with chemotherapy for the treatment of cervical adenocarcinoma cells in 2D and 3D cultures.Cancer cell international · 2022Article
- Luminescent Human iPSC-Derived Neurospheroids Enable Modeling of Neurotoxicity After Oxygen-glucose Deprivation.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2022Article
- 3D hanging spheroid plate for high-throughput CAR T cell cytotoxicity assay.Journal of nanobiotechnology · 2022Article
- Recent advances in 3D models of tumor invasion.Current opinion in biomedical engineering · 2021Article
- Spheroids and organoids as humanized 3D scaffold-free engineered tissues for SARS-CoV-2 viral infection and drug screening.Artificial organs · 2021Review
- Article
- Challenges of applying multicellular tumor spheroids in preclinical phase.Cancer cell international · 2021Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
We describe and share a device, methodology and image analysis algorithms, which allow up to 66 spheroids to be arranged into a gel-based array directly from a culture plate for downstream processing and analysis. Compared to processing individual samples, the technique uses 11-fold less reagents, saves time and enables automated imaging. To illustrate the power of the technology, we showcase applications of the methodology for investigating 3D spheroid morphology and marker expression and for in vitro safety and efficacy screens. First, spheroid arrays of 11 cell-lines were rapidly assessed for differences in spheroid morphology. Second, highly-positive (SOX-2), moderately-positive (Ki-67) and weakly-positive (βIII-tubulin) protein targets were detected and quantified. Third, the arrays enabled screening of ten media compositions for inducing differentiation in human neurospheres. Last, the application of spheroid microarrays for spheroid-based drug screens was demonstrated by quantifying the dose-dependent drop in proliferation and increase in differentiation in etoposide-treated neurospheres.
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