ArticleProceedings of the National Academy of Sciences of the United States of America1989
Molecular complementation of a genetic marker in Dictyostelium using a genomic DNA library.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 171 citations in OpenAlex.
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- An atypical MAPK regulates translocation of a GATA transcription factor in response to chemoattractant stimulation.Journal of cell science · 2022Article
- A deep coverage Dictyostelium discoideum genomic DNA library replicates stably in Escherichia coli.Genomics · 2015Article
- TOPSAN: a collaborative annotation environment for structural genomics.BMC bioinformatics · 2010Article
- An evolutionary analysis of lateral gene transfer in thymidylate synthase enzymes.Systematic biology · 2010Article
- Practical and theoretical advances in predicting the function of a protein by its phylogenetic distribution.Journal of the Royal Society, Interface · 2008Review
- Flavin-dependent thymidylate synthase ThyX activity: implications for the folate cycle in bacteria.Journal of bacteriology · 2007Article
- Developmental morphology and chemotactic responses are dependent on G alpha subunit specificity in Dictyostelium.Developmental biology · 2007Article
- Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics.BMC genetics · 2007Article
- Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium.Protein expression and purification · 2007Article
- Function and evolution of plasmid-borne genes for pyrimidine biosynthesis in Borrelia spp.Journal of bacteriology · 2006Article
- Archaeal Pyrococcus furiosus thymidylate synthase 1 is an RNA-binding protein.The Biochemical journal · 2006Article
- Sulfolobus tengchongensis spindle-shaped virus STSV1: virus-host interactions and genomic features.Journal of virology · 2005Article
- Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB.Molecular biology of the cell · 2005Article
- Parasexual genetics of Dictyostelium gene disruptions: identification of a ras pathway using diploids.BMC genetics · 2003Article
- Quod erat demonstrandum? The mystery of experimental validation of apparently erroneous computational analyses of protein sequences.Genome biology · 2001Article
- Article
- Dictyostelium IQGAP-related protein specifically involved in the completion of cytokinesis.The Journal of cell biology · 1997Article
- Subunit change in cytochrome c oxidase: identification of the oxygen switch in Dictyostelium.The EMBO journal · 1997Article
- Myosin light chain kinase (MLCK) gene disruption in Dictyostelium: a role for MLCK-A in cytokinesis and evidence for multiple MLCKs.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We constructed a partial Sau3A Dictyostelium genomic DNA library in a shuttle vector that replicates extrachromosomally in Dictyostelium cells. This library was used to complement Dictyostelium strain HPS400, which lacks thymidylate synthase activity and requires exogenous thymidine for growth. We have used a modified high-frequency transformation protocol that allows the introduction of the library into a sufficient number of Dictyostelium cells to select complementing plasmids. Using this approach, we have isolated a gene (Thy1) that complements the thymidine growth requirement of HPS400. The gene encodes a 1.2-kilobase RNA and the derived amino acid sequence shows no homology to thymidylate synthase, a protein highly conserved throughout evolution, or any other protein sequence in the data base examined. Thy1 provides an important selectable marker for transforming Dictyostelium cells. In addition, this work suggests that it will be possible to isolate genes that are essential for developmental processes in Dictyostelium by complementation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.