Evidence map›Paper›PMID 28133587›Full record

ArticleBioNanoScience2016

Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants.

Liliia R Nigmatullina, Margarita R Sharipova, Eugene V Shakirov

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioNanoScience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Telomere Length Variation in Model Bryophytes.Plants (Basel, Switzerland) · 2024
    Article
  3. Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Liliia R NigmatullinaLaboratory of Microbial Biotechnology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 9 Parizhskaya Communa str., Kazan, 420021, Russia.
Margarita R SharipovaLaboratory of Microbial Biotechnology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 9 Parizhskaya Communa str., Kazan, 420021, Russia.
Eugene V ShakirovLaboratory of Microbial Biotechnology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 9 Parizhskaya Communa str., Kazan, 420021, Russia; Department of Integrative Biology, University of Texas at Austin, Austin, 78712, TX, USA.

Funding

Identification and analysis of genetic determinants of natural telomere length variationR03AG052891 · NIA · UNIVERSITY OF TEXAS AT AUSTIN · PI SHAKIROV, EUGENE V · 2016 to 2017
$156k
NIA NIH HHS R03 AG052891
6 · The paper itself

Abstract

The length of telomeric DNA is often considered a cellular biomarker of aging and general health status. Several telomere length measuring assays have been developed, of which the most common is the Telomere Restriction Fragment (TRF) analysis, which typically involves the use of radioactively labeled oligonucleotide probes. While highly effective, this method potentially poses substantial health concerns and generates radioactive waste. Digoxigenin (DIG) alternatives to radioactive probes have been developed and used successfully in a number of assays. Here we optimize the DIG protocol to measure telomere length in the model plant

Indexed as

AgingDNANonradioactive telomere restriction fragment analysisPlantSouthern blot

Identifiers

PMID28133587
PMCPMC5268086

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.