ArticleFrontiers in microbiology2016
Preparation of MS2 Phage-Like Particles and Their Use As Potential Process Control Viruses for Detection and Quantification of Enteric RNA Viruses in Different Matrices.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Development, Characterization, and Validation of an MS2 Phage-Based Armored RNA Control for Schmallenberg Virus Detection.Pathogens (Basel, Switzerland) · 2026Article
- Viral RNA detections of enteric pathogens from caprine raw milk in Central Italy.Frontiers in microbiology · 2026Article
- Molecular uncovering of important helminth species in wild ruminants in the Czech Republic.Frontiers in veterinary science · 2025Article
- First Detection of Hepatitis E Virus RNA in Ovine Raw Milk from Herds in Central Italy.Foods (Basel, Switzerland) · 2024Article
- Sensitive Pathogen Detection and Drug Resistance Characterization Using Pathogen-Derived Enzyme Activity Amplified by LAMP or CRISPR-Cas.medRxiv : the preprint server for health sciences · 2024Article
- Virus-like particles derived from bacteriophage MS2 as antigen scaffolds and RNA protective shells.Nanomedicine (London, England) · 2024Review
- An alien parasite in a changing world -Frontiers in veterinary science · 2023Article
- One-plasmid double-expression system for preparation of MS2 virus-like particles packaging SARS-CoV-2 RNA.Frontiers in cellular and infection microbiology · 2023Article
- In vitro characterisation of the MS2 RNA polymerase complex reveals host factors that modulate emesviral replicase activity.Communications biology · 2022Article
- The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays.Parasites & vectors · 2021Article
- Evidence of Hepatitis E Virus in Goat and Sheep Milk.Viruses · 2020Article
- Viral Filtration Efficiency of Fabric Masks Compared with Surgical and N95 Masks.Pathogens (Basel, Switzerland) · 2020Article
- Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic.International journal of environmental research and public health · 2020Article
- Development and Evaluation of a Novel Armored RNA Technology Using Bacteriophage Qβ.Food and environmental virology · 2019Article
- Reverse Genetic Systems forMethods and protocols · 2019Article
- A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay.Scientific reports · 2019Article
- Preparation and Evaluation of Ribonuclease-Resistant Viral HIV RNA Standards Based on Armored RNA TechnologyIranian biomedical journal · 2018Article
- One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles.Scientific reports · 2017Article
- Virucidal Influence of Ionic Liquids on Phages P100 and MS2.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection and quantification of enteric RNA viruses is based on isolation of viral RNA from the sample followed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). To control the whole process of analysis and in order to guarantee the validity and reliability of results, process control viruses (PCV) are used. The present article describes the process of preparation and use of such PCV- MS2 phage-like particles (MS2 PLP) - in RT-qPCR detection and quantification of enteric RNA viruses. The MS2 PLP were derived from bacteriophage MS2 carrying a unique and specific
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