ArticleCytotechnology2017
Optimized expression of the antimicrobial protein Gloverin from Galleria mellonella using stably transformed Drosophila melanogaster S2 cells.
Article in Cytotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Process Intensification for Recombinant Marburg Virus Glycoprotein Production UsingEngineering in life sciences · 2025Article
- The VirtuousAntibiotics (Basel, Switzerland) · 2023Review
- The future of recombinant host defense peptides.Microbial cell factories · 2022Review
- Process intensification for the continuous production of an antimicrobial peptide in stably-transformed Sf-9 insect cells.Scientific reports · 2022Article
- Aeration and Shear Stress Are Critical Process Parameters for the Production of Oncolytic Measles Virus.Frontiers in bioengineering and biotechnology · 2019Article
- Single-cell cloning enables the selection of more productiveBiotechnology reports (Amsterdam, Netherlands) · 2018Article
- Dielectric Spectroscopy and Optical Density Measurement for the Online Monitoring and Control of Recombinant Protein Production in Stably Transformed Drosophila melanogaster S2 Cells.Sensors (Basel, Switzerland) · 2018Article
- Epigenetic Mechanisms Regulate Innate Immunity against Uropathogenic and Commensal-Like Escherichia coli in the Surrogate Insect Model Galleria mellonella.Infection and immunity · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antimicrobial proteins and peptides (AMPs) are valuable as leads in the pharmaceutical industry for the development of novel anti-infective drugs. Here we describe the efficient heterologous expression and basic characterization of a Gloverin-family AMP derived from the greater wax moth Galleria mellonella. Highly productive single-cell clones prepared by limiting dilution achieved a 100% increase in productivity compared to the original polyclonal Drosophila melanogaster S2 cell line. Comprehensive screening for suitable expression conditions using statistical experimental designs revealed that optimal induction was achieved using 600 µM CuSO
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Registered trials
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