ArticleMolecular microbiology2017
Insufficient levels of the nrdAB-encoded ribonucleotide reductase underlie the severe growth defect of the Δhda E. coli strain.
Article in Molecular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Establishment of titration-based control of DNA replication inScience advances · 2026Article
- The loop domains of the E. coli β sliding clamp are required for Hda-dependent inactivation of DnaA.Genetics · 2026Article
- Transcription-replication collisions trigger high-fidelity replication reset.Nucleic acids research · 2025Article
- Review
- Clues to transcription/replication collision-induced DNA damage: it was RNAP, in the chromosome, with the fork.FEBS letters · 2025Review
- Targeting synthesis of the Chromosome Replication Initiator Protein DnaA by antisense PNA-peptide conjugates inFrontiers in antibiotics · 2024Article
- Open Questions about the Roles of DnaA, Related Proteins, and Hyperstructure Dynamics in the Cell Cycle.Life (Basel, Switzerland) · 2023Review
- Negative feedback for DARS2-Fis complex by ATP-DnaA supports the cell cycle-coordinated regulation for chromosome replication.Nucleic acids research · 2021Article
- Elevated Levels of the Escherichia coliJournal of bacteriology · 2021Article
- The Mutant βJournal of bacteriology · 2021Article
- Hydroxyurea Induces a Stress Response That Alters DNA Replication and Nucleotide Metabolism in Bacillus subtilis.Journal of bacteriology · 2021Article
- Transcriptional Activity of the Bacterial Replication Initiator DnaA.Frontiers in microbiology · 2021Review
- A Novel Lysophosphatidic Acid Acyltransferase ofBiomolecules · 2020Article
- The bacterial cell cycle, chromosome inheritance and cell growth.Nature reviews. Microbiology · 2019Review
- Review
- Article
- Iron chelation increases the tolerance of Escherichia coli to hyper-replication stress.Scientific reports · 2018Article
- Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome replication.Nucleic acids research · 2018Article
- Countermeasures to survive excessive chromosome replication in Escherichia coli.Current genetics · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The ATP-bound form of the Escherichia coli DnaA replication initiator protein remodels the chromosomal origin of replication, oriC, to load the replicative helicase. The primary mechanism for regulating the activity of DnaA involves the Hda and β clamp proteins, which act together to dramatically stimulate the intrinsic DNA-dependent ATPase activity of DnaA via a process termed Regulatory Inactivation of DnaA. In addition to hyperinitiation, strains lacking hda function also exhibit cold sensitive growth at 30°C. Strains impaired for the other regulators of initiation (i.e., ΔseqA or ΔdatA) fail to exhibit cold sensitivity. The goal of this study was to gain insight into why loss of hda function impedes growth. We used a genetic approach to isolate 9 suppressors of Δhda cold sensitivity, and characterized the mechanistic basis by which these suppressors alleviated Δhda cold sensitivity. Taken together, our results provide strong support for the view that the fundamental defect associated with Δhda is diminished levels of DNA precursors, particularly dGTP and dATP. We discuss possible mechanisms by which the suppressors identified here may regulate dNTP pool size, as well as similarities in phenotypes between the Δhda strain and hda
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