Evidence map›Paper›PMID 28130631›Full record

ArticleApplied microbiology and biotechnology2017

Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter.

Verena Looser, Dominik Lüthy, Marcel Straumann, Katrin Hecht, Karel Melzoch, Karin Kovar

Open access · hybridAbstract read
In one paragraph

Article in Applied microbiology and biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 33 citations in OpenAlex.

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  9. Increase of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Verena LooserInstitute of Chemistry and Biotechnology, Zurich University of Applied Sciences (ZHAW), Campus Grüental, 8820, Wädenswil, Switzerland.
Dominik LüthyInstitute of Chemistry and Biotechnology, Zurich University of Applied Sciences (ZHAW), Campus Grüental, 8820, Wädenswil, Switzerland.
Marcel StraumannInstitute of Chemistry and Biotechnology, Zurich University of Applied Sciences (ZHAW), Campus Grüental, 8820, Wädenswil, Switzerland.
Katrin HechtInstitute of Chemistry and Biotechnology, Zurich University of Applied Sciences (ZHAW), Campus Grüental, 8820, Wädenswil, Switzerland.
Karel MelzochDepartment of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic.
Karin KovarInstitute of Chemistry and Biotechnology, Zurich University of Applied Sciences (ZHAW), Campus Grüental, 8820, Wädenswil, Switzerland. koka@zhaw.ch.
ZHAW Zurich University of Applied Sciences · CHUniversity of Chemistry and Technology, Prague · CZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As Pichia pastoris (syn. Komagataella sp.) yeast can secrete pure recombinant proteins at high rates, it is a desirable production system. The function of a novel synthetic variant of the AOX1 promoter was characterised comprehensively using a strain secreting Candida antarctica lipase B (CALB) as a model. A new time-saving approach was introduced to determine, in only one experiment, the hitherto unknown relationship between specific product formation rate (q

Indexed as

Promoter Regions, GeneticBatch Cell Culture TechniquesFermentationFungal ProteinsGlycerolKineticsLipaseMethanolPichiaRecombinant ProteinsFungal ProteinsGlycerolLipaselipase B, Candida antarcticaMethanolRecombinant ProteinsCandida antarctica lipase BMethanol-freePichia pastorisProduct formation kineticsSecretionSpecific productivity

Identifiers

PMID28130631
PMCPMC5380701
OpenAlexW2582162368

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.