ArticleNucleic acids research2017
AssemblX: a user-friendly toolkit for rapid and reliable multi-gene assemblies.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Vision and Development of a Design, Implementation, and Verification Automation (DIVA) Software Platform for DNA Construction.ACS synthetic biology · 2026Article
- In vivo pathway optimization in yeast via loxpsym-mediated shuffling of upstream activating sequences.Nucleic acids research · 2025Article
- PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes inACS synthetic biology · 2023Article
- A modular cloning (MoClo) toolkit for reliable intracellular protein targeting in the yeastMicrobial cell (Graz, Austria) · 2023Article
- Plant X-tender Toolbox for the Assembly and Expression of Multiple Transcriptional Units in Plants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Evolution-aided engineering of plant specialized metabolism.aBIOTECH · 2021Review
- Artificial Transcription Factors for Tuneable Gene Expression inFrontiers in bioengineering and biotechnology · 2021Article
- iFLinkC: an iterative functional linker cloning strategy for the combinatorial assembly and recombination of linker peptides with functional domains.Nucleic acids research · 2020Article
- Quantitative and Predictive Genetic Parts for Plant Synthetic Biology.Frontiers in plant science · 2020Review
- Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae.MicrobiologyOpen · 2019Article
- COMPASS for rapid combinatorial optimization of biochemical pathways based on artificial transcription factors.Nature communications · 2019Article
- Core Catalysis of the Reductive Glycine Pathway Demonstrated in Yeast.ACS synthetic biology · 2019Article
- L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast.Nature communications · 2018Article
- Plant X-tender: An extension of the AssemblX system for the assembly and expression of multigene constructs in plants.PloS one · 2018Article
- PhiReX: a programmable and red light-regulated protein expression switch for yeast.Nucleic acids research · 2017Article
- Synthetic Promoters and Transcription Factors for Heterologous Protein Expression inFrontiers in bioengineering and biotechnology · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly of large DNA constructs coding for entire pathways poses a major challenge in the field of synthetic biology. Here, we present AssemblX, a novel, user-friendly and highly efficient multi-gene assembly strategy. The software-assisted AssemblX process allows even unexperienced users to rapidly design, build and test DNA constructs with currently up to 25 functional units, from 75 or more subunits. At the gene level, AssemblX uses scar-free, overlap-based and sequence-independent methods, allowing the unrestricted design of transcriptional units without laborious parts domestication. The assembly into multi-gene modules is enabled via a standardized, highly efficient, polymerase chain reaction-free and virtually sequence-independent scheme, which relies on rare cutting restriction enzymes and optimized adapter sequences. Selection and marker switching strategies render the whole process reliable, rapid and very effective. The assembly product can be easily transferred to any desired expression host, making AssemblX useful for researchers from various fields.
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