ReviewMethods in cell biology2017
Best practices for germ-free derivation and gnotobiotic zebrafish husbandry.
Review in Methods in cell biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
95 citing papers in PubMed.
- Optimization of bacterial colonization in the gut of axenic and conventional zebrafish larvae using live food.Microbiology spectrum · 2026Article
- Estrogen impacts NOD2-dependent regulation of intestinal homeostasis.PLoS biology · 2026Article
- Article
- Article
- Integration of human microbiota (SIHUMIx) and zebrafish models reveals microbiome-mediated host responses to azoxystrobin.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- The Microbiota Shapes Central Nervous System Myelination in Early Life.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cross hybridization Inference for Phylogenetic Resolution (CIPHR)-FISH enables microbiome imaging with strain level taxonomic resolution.bioRxiv : the preprint server for biology · 2026Article
- Article
- Strengths and limitations ofGut microbes reports · 2026Review
- Imaging the impact of rotifer consumption on bacterial behaviors in the zebrafish gut.PloS one · 2026Article
- Article
- Recovery of infectious recombinant human norovirus using zebrafish embryos.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Novel and unscrutinized immune entities of the zebrafish gut.FEBS letters · 2025Review
- Long Term Culture of Germ-Free Zebrafish Using Gamma-Irradiated Feeds.bioRxiv : the preprint server for biology · 2025Article
- Generating gnotobiotic bivalves: a new method on Manila clam (Microbiology spectrum · 2025Article
- Characterizing trajectories of innate immune cells in larval zebrafish.bioRxiv : the preprint server for biology · 2025Article
- Influence of Housing, Sex, and Sampling Location on Taxonomy and Function of Adult Zebrafish Microbiomes.bioRxiv : the preprint server for biology · 2025Article
- Phollow reveals in situ phage transmission dynamics in the zebrafish gut microbiome at single-virion resolution.Nature microbiology · 2025Article
- Article
- ThemBio · 2025Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
All animals are ecosystems with resident microbial communities, referred to as microbiota, which play profound roles in host development, physiology, and evolution. Enabled by new DNA sequencing technologies, there is a burgeoning interest in animal-microbiota interactions, but dissecting the specific impacts of microbes on their hosts is experimentally challenging. Gnotobiology, the study of biological systems in which all members are known, enables precise experimental analysis of the necessity and sufficiency of microbes in animal biology by deriving animals germ-free (GF) and inoculating them with defined microbial lineages. Mammalian host models have long dominated gnotobiology, but we have recently adapted gnotobiotic approaches to the zebrafish (Danio rerio), an important aquatic model. Zebrafish offer several experimental attributes that enable rapid, large-scale gnotobiotic experimentation with high replication rates and exquisite optical resolution. Here we describe detailed protocols for three procedures that form the foundation of zebrafish gnotobiology: derivation of GF embryos, microbial association of GF animals, and long-term, GF husbandry. Our aim is to provide sufficient guidance in zebrafish gnotobiotic methodology to expand and enrich this exciting field of research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.