Evidence map›Paper›PMID 28129849›Full record

ReviewMethods in cell biology2017

In vivo imaging and quantification of regional adiposity in zebrafish.

J E N Minchin, J F Rawls

Open access · greenAbstract readReview
In one paragraph

Review in Methods in cell biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
15.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 37 citations in OpenAlex.

  1. Enteroendocrine cells wire the gut-brain vagal axis.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Transient fetal hyperglycemia reprograms adipogenesis, lipogenesis, and insulin signaling in zebrafish.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2026
    Article
  3. Review
  4. Article
  5. Follistatin like-1 (Adipocyte · 2024
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. A novel gnotobiotic experimental system for Atlantic salmon (Frontiers in cellular and infection microbiology · 2022
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

J E N MinchinUniversity of Edinburgh, Edinburgh, United Kingdom; Duke University, Durham, NC, United States.
J F RawlsDuke University, Durham, NC, United States.
Duke University · USUniversity of Edinburgh · GB

Funding

UNIV OF NORTH CAROLINA CLINICAL NUTRITION RESEARCH UNITP30DK056350 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Ian Michael Carroll · 1999 to 2026
$31.6M
Identifying genes required for digestive physiology and lipid metabolismR01DK093399 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI STEVEN A FARBER, John F Rawls · 2013 to 2026
$7.6M
Microbial Regulation of Host Nutrient MetabolismR01DK081426 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI RAWLS, JOHN F · 2008 to 2019
$3.9M
Organotin influences on assembly and obesogenic activity of the gut microbiotaR21ES023369 · NIEHS · DUKE UNIVERSITY · PI RAWLS, JOHN F · 2014 to 2015
$628k
Microbial regulation of host nutrient metabolism in gnotobiotic zebrafishK01DK073695 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI RAWLS, JOHN F · 2006 to 2009
$510k
Mechanisms of Adipose Depot Morphogenesis in ZebrafishR56DK091356 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI RAWLS, JOHN F · 2011 to 2011
$96k
British Heart FoundationNIDDK NIH HHS K01 DK073695NIDDK NIH HHS P30 DK056350NIDDK NIH HHS R01 DK081426NIDDK NIH HHS R01 DK093399NIDDK NIH HHS R56 DK091356NIEHS NIH HHS R21 ES023369
6 · The paper itself

Abstract

Adipose tissues (ATs) are lipid-rich structures that supply and sequester energy-dense lipid in response to the energy status of an organism. As such, ATs provide an organism energetic insurance during periods of adverse physiological burden. ATs are deposited in diverse anatomical locations, and excessive accumulation of particular regional ATs modulates disease risk. Therefore, a model system that facilitates the visualization and quantification of regional adiposity holds significant biomedical promise. The zebrafish (Danio rerio) has emerged as a new model system for AT research in which the entire complement of regional ATs can be imaged and quantified in live individuals. Here we present detailed methods for labeling adipocytes in live zebrafish using fluorescent lipophilic dyes, and for identifying and quantifying regional zebrafish ATs.

Indexed as

AdipocytesAdipose Tissue, WhiteAdiposityAnimalsLipidsMolecular ImagingObesityZebrafishLipidsAdipocyteAdipose tissueBody fat distributionIn vivo imagingLipid dropletZebrafish

Identifiers

PMID28129849
PMCPMC5497849
OpenAlexW2730040667

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.