ArticleStem cell research & therapy2017
Cord blood cell-derived iPSCs as a new candidate for chondrogenic differentiation and cartilage regeneration.
Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Chondrogenic potential of mesenchymal progenitors from somatic and cartilage-derived iPSCs is predicted by their transcriptomic signatures.Genes & diseases · 2026Article
- Clinical-grade iPSC-derived chondrogenic micropellets for treating advanced cartilage defects.Science advances · 2025Article
- Intelligent Manufacturing for Osteoarthritis Organoids.Cell proliferation · 2025Review
- The Effect of Nerve Growth Factor on Cartilage Fibrosis and Hypertrophy duringInternational journal of stem cells · 2025Article
- Combination of induced pluripotent stem cell-derived motor neuron progenitor cells with irradiated brain-derived neurotrophic factor over-expressing engineered mesenchymal stem cells enhanced restoration of axonal regeneration in a chronic spinal cord injury rat model.Stem cell research & therapy · 2024Article
- Induced pluripotent stem cells in cartilage tissue engineering: a literature review.Bioscience reports · 2024Review
- Ameliorative effects of undifferentiated and differentiated BM-MSCs in MIA-induced osteoarthritic Wistar rats: roles of NF-κB and MMPs signaling pathways.American journal of translational research · 2024Article
- Cell Therapy Approaches for Articular Cartilage Regeneration.Organogenesis · 2023Review
- Recent development in multizonal scaffolds for osteochondral regeneration.Bioactive materials · 2023Review
- A Fresh Glimpse into Cartilage Immune Privilege.Cartilage · 2022Review
- The role of the immune microenvironment in bone, cartilage, and soft tissue regeneration: from mechanism to therapeutic opportunity.Military Medical Research · 2022Review
- Generation of Functional Immortalized Human Corneal Stromal Stem Cells.International journal of molecular sciences · 2022Article
- Preferential stimulation of melanocytes by M2 macrophages to produce melanin through vascular endothelial growth factor.Scientific reports · 2022Article
- Review
- Review of the Current Trends in Clinical Trials Involving Induced Pluripotent Stem Cells.Stem cell reviews and reports · 2022Review
- Review
- Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing.Pharmaceutics · 2021Review
- Streamlining cell fate decisions during chondrogenesis.Nature reviews. Rheumatology · 2021Article
- Minicircles for Investigating and Treating Arthritic Diseases.Pharmaceutics · 2021Review
- iPSCs in Modeling and Therapy of Osteoarthritis.Biomedicines · 2021Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe native articular cartilage lacks the ability to heal. Currently, ex vivo expanded chondrocytes or bone marrow-derived mesenchymal stem cells are used to regenerate the damaged cartilage. With unlimited self-renewal ability and multipotency, human induced pluripotent stem cells (hiPSCs) have been highlighted as a new replacement cell source for cartilage repair. Still, further research is needed on cartilage regeneration using cord blood mononuclear cell-derived hiPSCs (CBMC-hiPSCs).
methodsHuman iPSCs were generated from CBMCs using the Sendai virus. The characterization of CBMC-hiPSCs was performed by various assays. Embryonic bodies (EBs) were obtained using CBMC-hiPSCs, and outgrowth cells were induced by plating the EBs onto a gelatin-coated plate. Expanded outgrowth cells were detached and dissociated for chondrogenic differentiation. Outgrowth cells were differentiated into chondrogenic lineage with pellet culture. Chondrogenic pellets were maintained for 30 days. The quality of chondrogenic pellets was evaluated using various staining and genetic analysis of cartilage-specific markers.
resultsReprogramming was successfully done using CBMCs. CBMC-hiPSCs (n = 3) showed high pluripotency and normal karyotype. Chondrogenic pellets were generated from the outgrowth cells derived from CBMC-hiPSC EBs. The generated chondrogenic pellets showed high expression of chondrogenic genetic markers such as ACAN, COMP, COL2A1, and SOX9. The production of extracellular matrix (ECM) proteins was confirmed by safranin O, alcian blue and toluidine blue staining. Expression of collagen type II and aggrecan was detected in the accumulated ECM by immunohistological staining. Chondrogenic pellets showed low expression of fibrotic and hypertrophic cartilage marker, collagen type I and X.
conclusionsThis study reveals the potential of CBMC-hiPSCs as a promising candidate for cartilage regeneration.
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