ArticleBiomaterials2017
Tissue engineering the mechanosensory circuit of the stretch reflex arc with human stem cells: Sensory neuron innervation of intrafusal muscle fibers.
Article in Biomaterials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Evaluating the peripheral nervous system pathology of Alzheimer's disease utilizing a functional human NMJ microphysiological system.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026Article
- Microphysiological systems for human aging research.Aging cell · 2024Review
- In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system.Scientific reports · 2022Article
- Engineering skeletal muscle: Building complexity to achieve functionality.Seminars in cell & developmental biology · 2021Review
- Cross-talk between motor neurons and myotubes via endogenously secreted neural and muscular growth factors.Physiological reports · 2021Article
- Methodological advances for studying gamma motor neurons.Current opinion in physiology · 2021Article
- Neuregulin 1 Drives Morphological and Phenotypical Changes in C2C12 Myotubes: TowardsFrontiers in cell and developmental biology · 2021Article
- Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease.Frontiers in physiology · 2021Review
- Frataxin gene editing rescues Friedreich's ataxia pathology in dorsal root ganglia organoid-derived sensory neurons.Nature communications · 2020Article
- Signals Orchestrating Peripheral Nerve Repair.Cells · 2020Review
- Engineered skeletal muscles for disease modeling and drug discovery.Biomaterials · 2019Review
- Strategies for using mathematical modeling approaches to design and interpret multi-organ microphysiological systems (MPS).APL bioengineering · 2019Review
- Recent Advances in Body-on-a-Chip Systems.Analytical chemistry · 2019Review
- The effects of neuregulin-1β on intrafusal muscle fiber formation in neuromuscular coculture of dorsal root ganglion explants and skeletal muscle cells.Skeletal muscle · 2018Article
- In Vitro Tissue-Engineered Skeletal Muscle Models for Studying Muscle Physiology and Disease.Advanced healthcare materials · 2018Review
- Multiorgan Microphysiological Systems for Drug Development: Strategies, Advances, and Challenges.Advanced healthcare materials · 2018Review
- A functional human motor unit platform engineered from human embryonic stem cells and immortalized skeletal myoblasts.Stem cells and cloning : advances and applications · 2018Article
- Challenges and Future Prospects on 3DFrontiers in bioengineering and biotechnology · 2018Review
- Functional analysis of human intrafusal fiber innervation by human γ-motoneurons.Scientific reports · 2017Article
- Age-Related Decline of Wrist Position Sense and its Relationship to Specific Physical Training.Frontiers in human neuroscience · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Muscle spindles are sensory organs embedded in the belly of skeletal muscles that serve as mechanoreceptors detecting static and dynamic information about muscle length and stretch. Through their connection with proprioceptive sensory neurons, sensation of axial body position and muscle movement are transmitted to the central nervous system. Impairment of this sensory circuit causes motor deficits and has been linked to a wide range of diseases. To date, no defined human-based in vitro model of the proprioceptive sensory circuit has been developed. The goal of this study was to develop a human-based in vitro muscle sensory circuit utilizing human stem cells. A serum-free medium was developed to drive the induction of intrafusal fibers from human satellite cells by actuation of a neuregulin signaling pathway. Both bag and chain intrafusal fibers were generated and subsequently validated by phase microscopy and immunocytochemistry. When co-cultured with proprioceptive sensory neurons derived from human neuroprogenitors, mechanosensory nerve terminal structural features with intrafusal fibers were demonstrated. Most importantly, patch-clamp electrophysiological analysis of the intrafusal fibers indicated repetitive firing of human intrafusal fibers, which has not been observed in human extrafusal fibers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.