ArticlePLoS pathogens2017
Characterization of Early-Phase Neutrophil Extracellular Traps in Urinary Tract Infections.
Article in PLoS pathogens, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Circulating Neutrophils Exhibit an Altered Immune Response in Chronic LUTS: An Image-Based Deep Learning Analysis.International urogynecology journal · 2026Article
- Decoding epithelial-immune cell crosstalk in uropathogenicFrontiers in microbiology · 2026Review
- Presence of neutrophil extracellular traps (NETs) in different types of human urinary tract infections (UTI). A pilot study.Frontiers in immunology · 2026Article
- Article
- Clinical isolates ofFrontiers in immunology · 2025Article
- Uropathogens and their antibiotic susceptibility patterns among diabetic patients at st. john of god hospital, duayaw nkwanta, Ghana: a cross-sectional study.Health science reports · 2024Article
- A Dynamic Interplay of Innate Immune Responses During Urinary Tract Infection.Immune network · 2024Review
- Organ agar serves as physiologically relevant alternative forInfection and immunity · 2023Article
- Mitochondria and cytochrome components released into the plasma of severe COVID-19 and ICU acute respiratory distress syndrome patients.Clinical proteomics · 2023Article
- Impaired Abcb1a function and red meat in a translational colitis mouse model induces inflammation and alters microbiota composition.Frontiers in medicine · 2023Article
- Neutrophil extracellular traps in urinary tract infection.Frontiers in pediatrics · 2023Article
- Timing is everything: impact of development, ageing and circadian rhythm on macrophage functions in urinary tract infections.Mucosal immunology · 2022Review
- Study of the Role of the Tyrosine Kinase Receptor MerTK in the Development of Kidney Ischemia-Reperfusion Injury in RCS Rats.International journal of molecular sciences · 2021Article
- Article
- To Trap a Pathogen: Neutrophil Extracellular Traps and Their Role in Mucosal Epithelial and Skin Diseases.Cells · 2021Review
- Protein and Microbial Biomarkers in Sputum Discern Acute and Latent Tuberculosis in Investigation of Pastoral Ethiopian Cohort.Frontiers in cellular and infection microbiology · 2021Article
- Urethral Catheter Biofilms Reveal Plasticity in Bacterial Composition and Metabolism and Withstand Host Immune Defenses in Hypoxic Environment.Frontiers in medicine · 2021Article
- Mammalian histones facilitate antimicrobial synergy by disrupting the bacterial proton gradient and chromosome organization.Nature communications · 2020Article
- Kidney dendritic cells: fundamental biology and functional roles in health and disease.Nature reviews. Nephrology · 2020Review
- Antimicrobial Microwebs of DNA-Histone Inspired from Neutrophil Extracellular Traps.Advanced materials (Deerfield Beach, Fla.) · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Neutrophils have an important role in the antimicrobial defense and resolution of urinary tract infections (UTIs). Our research suggests that a mechanism known as neutrophil extracellular trap (NET) formation is a defense strategy to combat pathogens that have invaded the urinary tract. A set of human urine specimens with very high neutrophil counts had microscopic evidence of cellular aggregation and lysis. Deoxyribonuclease I (DNase) treatment resulted in disaggregation of such structures, release of DNA fragments and a proteome enriched in histones and azurophilic granule effectors whose quantitative composition was similar to that of previously described in vitro-formed NETs. The effector proteins were further enriched in DNA-protein complexes isolated in native PAGE gels. Immunofluorescence microscopy revealed a flattened morphology of neutrophils associated with decondensed chromatin, remnants of granules in the cell periphery, and myeloperoxidase co-localized with extracellular DNA, features consistent with early-phase NETs. Nuclear staining revealed that a considerable fraction of bacterial cells in these structures were dead. The proteomes of two pathogens, Staphylococcus aureus and Escherichia coli, were indicative of adaptive responses to early-phase NETs, specifically the release of virulence factors and arrest of ribosomal protein synthesis. Finally, we discovered patterns of proteolysis consistent with widespread cleavage of proteins by neutrophil elastase, proteinase 3 and cathepsin G and evidence of citrullination in many nuclear proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.